XPV/DNA polymerase eta Antibody - BSA Free

Novus Biologicals | Catalog # NB100-60423

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all XPV Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 640 and 690 of human polymerase (DNA directed), eta using the numbering given in entry NP_006493.1 (GeneID 5429).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XPV/DNA polymerase eta Antibody - BSA Free

Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60423]

Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60423]

Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60423] - Detection of Human PolH by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PolH antibody NB100-60423 used for IP at 3 mcg/mg lysate. PolH was also immunoprecipitated by rabbit anti-PolH antibody NB100-60424, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for XPV/DNA polymerase eta Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XPV

DNA polymerase eta is specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. It also plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. It targets POLI to replication foci (referenced from swissprot).

Long Name

Xeroderma Pigmentosum Variant Type Protein

Alternate Names

DNA Polymerase, eta, POLH, RAD30A

Entrez Gene IDs

5429 (Human)

Gene Symbol

POLH

UniProt

NP_006493.1 (Human)

Additional XPV Products

Product Documents for XPV/DNA polymerase eta Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for XPV/DNA polymerase eta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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