Yoda 1

Tocris Bioscience | Catalog # 5586

Piezo1 channel activator
Tocris Bioscience

Key Product Details

Description

Piezo1 channel activator
View all Piezo Channel Activators »

Product Description

Yoda 1 is a selective activator of mouse and human mechanosensitive channel piezo1. In HEK cells, Yoda 1 slows the inactivation phase of transient currents, sensitizes Piezo1 to activation by pressure, and partially activates channels in the absence of external pressure. In MDCK cells, Yoda 1 induces Ca2+-dependent chromatin hypercondensation and alters Ca2+-dependent myosin contractility, leading to nuclear shrinkage in cells. In red blood cells from sickle cell anemia (SCA) patients, Yoda 1 increases intracellular Ca2+ and phosphatidylserine exposure, which leads to KCa3.1 channel mediated Ca2+ influx and K+ and water efflux, causing shrinkage of the cells.

Product Specifications for Yoda 1

Molecular Weight

355.27

Formula

C13H8Cl2N4S2

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

2-[5-[[(2,6-Dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]pyrazine

CAS Number

448947-81-7

PubChem ID

2746822

InChI Key

BQNXBSYSQXSXPT-UHFFFAOYSA-N

SMILES

ClC1=C(CSC2=NN=C(C3=NC=CN=C3)S2)C(Cl)=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.11 20

Preparing Stock Solutions for Yoda 1

The following data is based on the product molecular weight 355.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 14.07 mL 70.37 mL 140.74 mL
1 mM 2.81 mL 14.07 mL 28.15 mL
2 mM 1.41 mL 7.04 mL 14.07 mL
10 mM 0.28 mL 1.41 mL 2.81 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 184 publications citing the usage of this product.

Product Documents for Yoda 1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Yoda 1

For research use only

Customer Reviews for Yoda 1 (2)

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  • Stimulation of Piezo1 activity
    Name: Vladislav Chubinskiy-Nadezhdin
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: 3T3B-SV40
    Verified Customer | Posted 08/16/2019
    Used in patch-clamp, cell migration assay (1-30 uM)
  • A potent piezo1 receptor activator
    Name: Hyun Choi
    Species: Rat
    Assay Type: Ex Vivo
    Verified Customer | Posted 02/21/2018
    We used to stimulate piezo1 receptor at around 1-10microM

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