YY1 Antibody

Novus Biologicals | Catalog # NBP2-20933

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (97%), Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all YY1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human YY1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (80%), Chicken (84%).

Localization

Nucleus matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for YY1 Antibody

Immunoprecipitation: YY1 Antibody [NBP2-20933]

Immunoprecipitation: YY1 Antibody [NBP2-20933]

Immunoprecipitation: YY1 Antibody [NBP2-20933] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of YY1protein by 2 ug of YY1 antibody 10% SDS-PAGE The immunoprecipitated YY1 protein was detected by YY1 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
YY1 Antibody

Western Blot: YY1 Antibody [NBP2-20933] -

YY1 antibody detects YY1 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
10 % SDS-PAGE
YY1 antibody (NBP2-20933) dilution: 1:1000

Applications for YY1 Antibody

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: YY1

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq]

Long Name

Yin-Yang-1

Alternate Names

DELTA, NF-E1, UCRBP

Gene Symbol

YY1

UniProt

P25490

Additional YY1 Products

Product Documents for YY1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for YY1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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