Z-D(OMe)E(OMe)VD(OMe)-FMK (Caspase 3/7 Inhibitor)

Novus Biologicals | Catalog # NBP2-29396

Novus Biologicals
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Product Specifications

Application Notes

This inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate the free carboxyl groups.

Formulation, Preparation, and Storage

Preparation Method

Formula: C30H41O12N4F
Molecular Weight: 668
Mass Spec: M+1=669.3.
Chromatography: EtOAc:9, Hex: 1, Single Spot, Rf:0.5

Formulation

Form: White Solid
Make a stock solution of 20 mM in high purity DMSO (>99.9%).

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Z-D(OMe)E(OMe)VD(OMe)-FMK

Members of the caspase family play key roles in apoptosis and inflammation. Z-D(OMe)E(OMe)VD(OMe)-FMK (ZDEVD- FMK) is a cell-permeable caspase peptide inhibitor that irreversibly binds to the catalytic site of caspases proteases, and inhibits caspase-mediated apoptosis by preventing caspase activity (reviewed in 1-3). Caspase inhibitors, like Z-DEVD-FMK, with the peptide recognition sequence DEVD are potent inhibitors of caspase-3 and were first described as caspase-3 inhibitors. It is now known that caspase inhibitors with the peptide recognition sequence DEVD also inhibit other caspases, albeit at higher concentrations. However, Z-DEVD-FMK and other inhibitors with the DEVD peptide recognition sequence continue to be denoted as caspase-3 specific inhibitors by a number of sources. The Z-D(OMe)E(OMe)VD(OMe)-FMK (Z-DEVD-FMK) peptide is O-methylated in the P4, P3 and P1 positions providing enhanced stability and increased cell permeability. Z-DEVD-FMK is typically used in assays to inhibit apoptosis. Z-DEVD-FMK has been used in many different types of apoptosis assays and published extensively; for example, a PubMed search reveals over 300 references citing Z-DEVD-FMK between 1999 and 2003. Users may want to consult the literature for additional information regarding applications for Z-DEVD-FMK.

Alternate Names

Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK, Z-D(OMe)E(OMe)VD(OMe)-FMK, Z-DOMeEOMeVDOMe-FMK

Additional Z-D(OMe)E(OMe)VD(OMe)-FMK Products

Product Documents for Z-D(OMe)E(OMe)VD(OMe)-FMK (Caspase 3/7 Inhibitor)

Certificate of Analysis

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Product Specific Notices for Z-D(OMe)E(OMe)VD(OMe)-FMK (Caspase 3/7 Inhibitor)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for Z-D(OMe)E(OMe)VD(OMe)-FMK (Caspase 3/7 Inhibitor) (NBP2-29396):

Product Handling - Assay Method (NBP2-29396):
Materials:
Dissolve 1mg of Z-D(OMe)E(OMe)VD(OMe)-FMK in DMSO to get appropriate concentration:
75 ul DMSO = 20mM
150 ul DMSO = 10 mM
300 ul DMSO = 5 mM

Method:
Add 2 ul of above stock solutions to 1 ml of culture medium containing cells to give final DMSO concentration of
0.2%. Levels of DMSO above this may cause some cellular toxicity thus masking the effect of the ICE-protease
inhibitors. Two microliter of 10mM stock solution in 1 ml medium = 20 uM final Z-D(OMe)E(OMe)VD(OMe)-FMK
concentration. Caspase inhibitors have been successfully used in tissue cultures to inhibit apoptosis at final working
concentrations of approximately 50 nM to 100 uM. The variability reflects cell type and method. Investigators should
determine the optimal concentration empirically.