Catalog Number: 2163
Chemical Name: Benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone
Biological Activity
Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC50 = 0.0015 - 5.8 mM). Active in vivo.
Technical Data
  • M.Wt:
  • Formula:
  • Sequence:

    (Modifications: Val-1 = Z-Val, Asp-3 = (OMe)-fluoromethylketone)

  • Solubility:
    Soluble to 9.35 mg/ml in DMSO
  • Storage:
    Store at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32.
    Slee et al.
    Biochem.J., 1996;315:21
  2. ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-α.
    Kunstle et al.
    Immunol.Lett., 1997;55:5
  3. Inhibition of human caspases by peptide-based and macromolecular inhibitors.
    Garcia-Calvo et al.
    J.Biol.Chem., 1998;273:32608
  4. Processing/activation of caspases, -3 and -7 and -8 but not caspase-2, in the induction of apoptosis in B-chronic lyphocytic leukemia cells.
    King et al.
    Leukemia, 1998;12:1553

The citations listed below are publications that use Tocris products. Selected citations for Z-VAD-FMK include:

18 Citations: Showing 1 - 10
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  1. Methylsulfonylmethane Induces p53 Independent Apoptosis in HCT-116 Colon Cancer Cells
    Authors: Karabay Et al.
    International Journal of Molecular Sciences 2016;17:1123
  2. The NLRP3 inflammasome is activated by nanoparticles through ATP, ADP and adenosine.
    Authors: Baron Et al.
    Mol Cell Biol 2015;6:e1629
  3. NF-κB signaling is required for XBP1 (unspliced and spliced)-mediated effects on antiestrogen responsiveness and cell fate decisions in breast cancer.
    Authors: Hu Et al.
    Clin Cancer Res 2015;35:379
  4. Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells.
    Authors: Amantini Et al.
    Cell Death Dis 2015;2:395
  5. Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation.
    Authors: Bonnefond Et al.
    Oncoscience 2015;20:535
  6. A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells.
    Authors: Silva Et al.
    Apoptosis 2015;14:1070
  7. Parasympathetic innervation regulates tubulogenesis in the developing salivary gland.
    Authors: Nedvetsky Et al.
    Dev Cell 2014;30:449
  8. A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells.
    Authors: Hight Et al.
    Evid Based Complement Alternat Med 2014;20:2126
  9. The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells.
    Authors: Sagher Et al.
    Cancer Cell Int 2014;14:75
  10. The mTORC2 component rictor contributes to cisplatin resistance in human ovarian cancer cells.
    Authors: Im-aram Et al.
    PLoS One 2013;8:e75455
  11. A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells.
    Authors: Kim Et al.
    Cell Death Dis 2013;4:e750
  12. A drug repositioning approach identifies tricyclic antidepressants as inhibitors of small cell lung cancer and other neuroendocrine tumors.
    Authors: Jahchan Et al.
    Cancer Discov 2013;3:1364
  13. Active Constituents from Liriope platyphylla Root against Cancer Growth In Vitro.
    Authors: Wang Et al.
    Mar Drugs 2013;2013:857929
  14. Cytotoxic effect of clerosterol isolated from Codium fragile on A2058 human melanoma cells.
    Authors: Kim Et al.
    Mol Cancer Res 2013;11:418
  15. ATM and MET kinases are synthetic lethal with nongenotoxic activation of p53.
    Authors: Sullivan Et al.
    Cell Cycle 2012;8:646
  16. Glucose-regulated protein 78 controls cross-talk between apoptosis and autophagy to determine antiestrogen responsiveness.
    Authors: Cook Et al.
    Oncotarget 2012;72:3337
  17. Calpain-mediated processing of p53-associated parkin-like cytoplasmic protein (PARC) affects chemosensitivity of human ovarian cancer cells by promoting p53 subcellular trafficking.
    Authors: Woo Et al.
    J Biol Chem 2012;287:3963
  18. Glioma regression in vitro and in vivo by a suicide combined treatment.
    Authors: García-Escudero Et al.
    Cancer Res 2008;6:407
Expand to show all 18 Citations

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