Chemical Name: Benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone
Biological ActivityZ-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC50 = 0.0015 - 5.8 mM). Active in vivo.
(Modifications: Val-1 = Z-Val, Asp-3 = (OMe)-fluoromethylketone)
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32.
Slee et al.
ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-α.
Kunstle et al.
Inhibition of human caspases by peptide-based and macromolecular inhibitors.
Garcia-Calvo et al.
Processing/activation of caspases, -3 and -7 and -8 but not caspase-2, in the induction of apoptosis in B-chronic lyphocytic leukemia cells.
King et al.
Citations for Z-VAD-FMK
The citations listed below are publications that use Tocris products. Selected citations for Z-VAD-FMK include:
32 Citations: Showing 1 - 10
Circulating Cell-Free mtDNA Contributes to AIM2 Inflammasome-Mediated Chronic Inflammation in Patients with Type 2 Diabetes.
Authors: Bae Et al.
Necroptosis in primate luteolysis: a role for ceramide.
Authors: Bagnjuk Et al.
Cell Death Discov 2019;5:67
Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage.
Authors: Uderhardt Et al.
ONC201 kills breast cancer cells in vitro by targeting mitochondria.
Authors: Greer Et al.
Phosphatidylserine is a marker for axonal debris engulfment but its exposure can be decoupled from degeneration.
Authors: Shacham-Silverberg Et al.
Cell Death Dis 2018;9:1116
US6 Gene Deletion in Herpes Simplex Virus Type 2 Enhances Dendritic Cell Function and T Cell Activation.
Authors: Retamal-Díaz Et al.
Front Immunol 2017;8:1523
Methylsulfonylmethane Induces p53 Independent Apoptosis in HCT-116 Colon Cancer Cells
Authors: Karabay Et al.
International Journal of Molecular Sciences 2016;17:1123
Guadecitabine (SGI-110) priming sensitizes hepatocellular carcinoma cells to oxalip.
Authors: Kuang Et al.
Mol Oncol 2015;9:1799
The NLRP3 inflammasome is activated by nanoparticles through ATP, ADP and adenosine.
Authors: Baron Et al.
Mol Cell Biol 2015;6:e1629
NF-κB signaling is required for XBP1 (unspliced and spliced)-mediated effects on antiestrogen responsiveness and cell fate decisions in breast cancer.
Authors: Hu Et al.
Clin Cancer Res 2015;35:379
Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells.
Authors: Amantini Et al.
Cell Death Dis 2015;2:395
Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation.
Authors: Bonnefond Et al.
Human ACAP2 is a homolog of C. elegans CNT-1 that promotes apoptosis in cancer cells.
Authors: Sullivan Et al.
Cell Cycle 2015;14:1771
A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells.
Authors: Silva Et al.
mTORC1-Induced HK1-Dependent Glycolysis Regulates NLRP3 Inflammasome Activation.
Authors: Moon Et al.
Cell Rep 2015;12:102
Recombinant adeno-associated virus utilizes host cell nuclear import machinery to enter the nucleus.
J Virol 2014;88:4132
The ethyl acetate fraction of Sargassum muticum attenuates ultraviolet B radiation-induced apoptotic cell death via regulation of MAPK- and caspase-dependent signaling pathways in human HaCaT keratinocytes.
Authors: Piao Et al.
Pharm Biol 2014;52:1110
Parasympathetic innervation regulates tubulogenesis in the developing salivary gland.
Authors: Nedvetsky Et al.
Dev Cell 2014;30:449
A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells.
Authors: Hight Et al.
Evid Based Complement Alternat Med 2014;20:2126
The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells.
Authors: Sagher Et al.
Cancer Cell Int 2014;14:75
Role of ROS and HMGB1 in contact allergen-induced IL-18 production in human keratinocytes.
Authors: Galbiati Et al.
J Invest Dermatol 2014;134:2719
The mTORC2 component rictor contributes to cisp. resistance in human ovarian cancer cells.
Authors: Im-aram Et al.
PLoS One 2013;8:e75455
A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells.
Authors: Kim Et al.
Cell Death Dis 2013;4:e750
A drug repositioning approach identifies tricyclic antidepressants as inhibitors of small cell lung cancer and other neuroendocrine tumors.
Authors: Jahchan Et al.
Cancer Discov 2013;3:1364
Active Constituents from Liriope platyphylla Root against Cancer Growth In Vitro.
Authors: Wang Et al.
Mar Drugs 2013;2013:857929
Cytotoxic effect of clerosterol isolated from Codium fragile on A2058 human melanoma cells.
Authors: Kim Et al.
Mol Cancer Res 2013;11:418
Annexin A1 modulates natural and glucocorticoid-induced resolution of inflammation by enhancing neutrophil apoptosis.
Authors: Vago Et al.
J Leukoc Biol 2012;92:249
ATM and MET kinases are synthetic lethal with nongenotoxic activation of p53.
Authors: Sullivan Et al.
Cell Cycle 2012;8:646
Glucose-regulated protein 78 controls cross-talk between apoptosis and autophagy to determine antiestrogen responsiveness.
Authors: Cook Et al.
Calpain-mediated processing of p53-associated parkin-like cytoplasmic protein (PARC) affects chemosensitivity of human ovarian cancer cells by promoting p53 subcellular trafficking.
Authors: Woo Et al.
J Biol Chem 2012;287:3963
GS28 Protects Neuronal Cell Death Induced by Hydrogen Peroxide under Glutathione-Depleted Condition.
Authors: Lee Et al.
Korean J Physiol Pharmacol 2011;15:149
Glioma regression in vitro and in vivo by a suicide combined treatment.
Authors: García-Escudero Et al.
Cancer Res 2008;6:407
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We pretreated to cultured microglia to inhibit caspases activity at 20microM
AML12 hepatocytes were cultured and were per-treated with Z-VAD-FMK.