ZEB1 Antibody (BLR102H)

Novus Biologicals | Catalog # NBP3-14694

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR102H
View all ZEB1 Primary Antibodies »

Product Specifications

Immunogen

between 1074 and 1124 (C-term)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for ZEB1 Antibody (BLR102H)

Immunoprecipitation: ZEB1 Antibody (BLR102H) [NBP3-14694]

Immunoprecipitation: ZEB1 Antibody (BLR102H) [NBP3-14694]

Immunoprecipitation: ZEB1 Antibody [NBP3-14694] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-ZEB1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. ZEB1 was alsoimmunoprecipitated by rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.

Applications for ZEB1 Antibody (BLR102H)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: ZEB1

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Gene Symbol

ZEB1

Additional ZEB1 Products

Product Documents for ZEB1 Antibody (BLR102H)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ZEB1 Antibody (BLR102H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ZEB1 Antibody (BLR102H)

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  • Q: In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?

    A: ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.

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