Zhangfei Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94859

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Zhangfei Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human CREBZF (NP_001034707.1). MRHSLTKLLAASGSNSPTRSESPEPAATCSLPSDLTRAAAGEEETAAAGSPGRKQQFGDEGELEAGRGSRGGVAVRAPSPEEMEEEAIASLPGEETEDMDFLSGLELADLLDPRQPDWHLDPGLSSPGPLSSSGGGSDSGGLWRGDDDDEAAAAEMQRFSDLLQRLLNGIGGCSSSSDSGSAEKRRRKSPGGGGGGGSGNDNNQAATKSPRKAAAAAARLNRLKKKEYVMGLESRVRGLAAENQELRAENRELGKRVQALQEESRYLRAVLANETGLARLLSRLSGVGLRLTTSLFRDSPAGDHDYALPVGKQKQDLLEEDDSAGGVCLHVDKDKVSVEFCSACARKASSSLKM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Zhangfei Antibody - Azide and BSA Free

Western Blot: Zhangfei AntibodyAzide and BSA Free [NBP2-94859]

Western Blot: Zhangfei AntibodyAzide and BSA Free [NBP2-94859]

Western Blot: Zhangfei Antibody [NBP2-94859] - Analysis of extracts of various cell lines, using Zhangfei.Exposure time: 1s.
Zhangfei Antibody - Azide and BSA Free

Western Blot: Zhangfei Antibody - Azide and BSA Free [NBP2-94859] -

SMILE and MITF are reciprocally regulated by alpha -MSH in B16F10 mouse melanoma cells. Cells were treated with 1 uM alpha -MSH in a time-dependent manner. (a) Protein levels of MITF, melanogenic enzymes, and SMILE. (b) mRNA levels of genes encoding SMILE and MITF. * p < 0.05 vs. no treatment of alpha -MSH by one-way ANOVA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36499416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Zhangfei Antibody - Azide and BSA Free

Western Blot: Zhangfei Antibody - Azide and BSA Free [NBP2-94859] -

SMILE overexpression downregulates the expression of melanogenic genes in B16F10 cells. Cells were infected with Ad-GFP or Ad-SMILE for 48 h and then, treated with 1 uM alpha -MSH for 1 h or 24 h. (a) Melanin contents. (b) Cellular tyrosinase activity. (c) mRNA levels of melanogenic genes. (d) Protein levels of SMILE and melanogenic genes. Data are mean +/- SEM. * p < 0.05 vs. Ad-GFP; #p < 0.05 vs. Ad-GFP + alpha -MSH by one-way ANOVA (a). * p < 0.05 vs. Ad-GFP (b,c) by Student’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36499416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Zhangfei Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Zhangfei

Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cellsinfected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation byCREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes inCREBZF-expressing cells

Alternate Names

CREB/ATF bZIP transcription factor, FLJ94018, HCF-binding transcription factor Zhangfei, Host cell factor-binding transcription factor Zhangfei, SHP-interacting leucine zipper protein, SMILE, Zhangfei

Gene Symbol

CREBZF

Additional Zhangfei Products

Product Documents for Zhangfei Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Zhangfei Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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