Ready-to-Use Cytokines for Simplified Workflows |

Ready-to-Use Animal-Free GMP and RUO Proteins

Protein Name	Liquid		Process-Sized
Protein Name	RUO Vial	GMP Vial	GMP Vial (Lyophilized)	GMP ProPak^™ (Liquid in Bag)
IL-2	50 μg, 1 mg	50 μg, 1 mg		10 µg, 150k IU, or 3M IU
IL-7	25 μg	25 μg	10 µg	10 µg
IL-15	25 μg	25 μg	10 µg	10 µg
IL-21	25 μg, 1 mg	25 μg, 1 mg

Liquid formulations for these proteins are currently available to ship to the United States, Canada, and Europe. We are working to expand shipping to other parts of the world.

Liquid Cytokines: Eliminate Reconstitution Steps

Designed to simplify your complex processes, liquid frozen cytokines streamline your workflow, simplify your protocol, and achieve greater research efficiencies by eliminating the reconstitution step. This removes the risk of errors associated with reconstitution plus eliminates the need to maintain a sterile reconstitution buffer. Simply thaw and add to your cell culture.

Protein stability is paramount to ensure safety, bioactivity, and minimal variability. With this in mind, we have optimized protein concentrations and conducted extensive stability testing. You can rest assured of the potency and effectiveness of the liquid cytokines over time, ensuring consistent performance and reliable outcomes for your cell therapy.

Process-Sized Cytokines: Eliminate Aliquoting Steps

Process-sized vials remove the need to aliquot your cytokines, allowing for higher patient safety and reduced hands-on technician time. These single-use vials have been process-optimized and are compatible with any immune cell manufacturing system, including ScaleReady G-Rex^® bioreactors. Simply use a syringe to reconstitute cytokines in the vial and add to your cell culture. 

Simplify your cell manufacturing workflow
Reduce manual touchpoints
Eliminate aliquoting variability

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ProPak: The Benefits of Liquid and Process-Sized GMP Cytokines

ProPak GMP cytokines offer a "best of both" approach: liquid cytokines in process sizes, with the additional benefit of weldable bags supporting a closed process workflow. GMP IL-2, IL-7, and IL-15 are delivered through process-sized bags with weldable tubing and are compatible with bagged media for use in a 1 L bioreactor, such as the G-Rex.

Learn how to add these GMP cytokines to your media in the ProPak User Manual.

Learn More About Closed System Solutions

Cytokine Titration Data

The presence of IL-15 is necessary to maintain T cell growth.
Cells do not expand in the absence of both cytokines.

The presence of IL-7 necessary to maintain CCR7 expression.
Characterizing cells by CD45RA and CCR7 revealed minimal difference in T cell memory phenotype.

Overdosing of IL-15 can decrease expression of the key memory marker CD127, especially in CD8⁺ T cells.
CD127 expression was more sensitive to IL-15 titration that CCR7 was.

CD127 expression is relatively stable independent of IL-7 titration.

In-house scientists determined the concentrations of IL-7 and IL-15 that yield the optimum balance of T cell expansion and memory phenotype. Based on these findings, we now offer process-sized cytokines, enabling you to generate your desired phenotype while requiring less protein and producing less waste. For a demonstration of a hands-free T cell expansion workflow, check out the G-Rex Fill-and-Forget Workflow Application Note.

Equivalent Bioactivity Between Liquid and Lyophilized Cytokines

Direct comparisons of the liquid frozen (blue circles) and lyophilized (red squares) formulations of GMP IL-2, IL-7, and IL-15. All 3 cytokines were tested in cell proliferation assays with no difference in bioactivity observed for any of the cytokines. IL-2 was tested on CTLL-2 mouse cytotoxic T cells, IL-7 was tested on PHA-activated human peripheral blood lymphocytes, and IL-15 was tested on MO7e human megakaryocytic leukemic cells.

Equivalent T Cell Expansion and Phenotype Between Liquid and Lyophilized Cytokines

Primary human T cell expansion was evaluated using both liquid format and lyophilized cytokines. Cells were expanded in G-Rex^® 6M well plates using complete GMP Human T Cell Expansion Media supplemented with either 200 IU/mL GMP IL-2 or a combination of 10 ng/mL of both GMP IL-7 and GMP IL-15 in both liquid and lyophilized formats. After 9 days of expansion, cells were assessed for fold expansion and viability (left; data labels are mean % viability) as well as for memory phenotype using flow cytometry (right).

Specifications of Liquid and Lyophilized Cytokines

IL-2	Liquid	Lyophilized
Formulation	0.50 mg/mL, 0.2 μm filtered solution in sodium acetate	Lyophilized from a 0.2 μm filtered solution in sodium acetate with Trehalose
Stability	A minimum of 6 months when stored at ≤ -65 °C as supplied	A minimum of 12 months when stored at ≤ -20 °C as supplied
Pack Size	10 µg, 150k IU, or 3M IU bag, 50 μg, 1 mg	50 μg, 1 mg

IL-7	Liquid	Lyophilized
Formulation	0.50 mg/mL, 0.2 μm filtered solution in PBS	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
Stability	A minimum of 6 months when stored at ≤ -65 °C as supplied	A minimum of 12 months when stored at ≤ -20 °C as supplied
Pack Size	10 µg bag (1L process size), 25 μg	10 µg (1L process size), 25 μg, 1 mg

IL-15	Liquid	Lyophilized
Formulation	0.50 mg/mL, 0.2 μm filtered solution in PBS	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
Stability	A minimum of 6 months when stored at ≤ -65 °C as supplied	A minimum of 12 months when stored at ≤ -20 °C as supplied
Pack Size	10 µg bag (1L process size), 25 μg	10 µg (1L process size), 25 μg, 1 mg

IL-21	Liquid	Lyophilized
Formulation	0.50 mg/mL, 0.2 μm filtered solution in PBS	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
Stability	A minimum of 6 months when stored at ≤ -65 °C as supplied	A minimum of 12 months when stored at ≤ -20 °C as supplied
Pack Size	25 μg, 1 mg	25 μg, 1 mg

Stability data for liquid frozen cytokines is available on request.

Simplify Your Immune Cell Workflow With Ready-to-Use Protein Formats