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Technical Note: R&D Systems Cell Selection Kits for Cancer-associated Cells
MagCellect™ Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit
R&D Systems offers several kits that are designed to isolate tumor associated cell populations, including a breast cancer stem cell isolation kit and multiple other kits that isolate cells expressing specific cancer-related markers. The MagCellect Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit (Catalog # MAGH111) was designed to isolate a small population of human breast cancer cells that have the unique ability to form new tumors in mice.1 This kit utilizes a two-step procedure that combines negative and positive selection techniques. CD24low/– cells are initially enriched by negative selection whereby unwanted CD24+ cells are tagged and magnetically removed. CD44+ cells are subsequently isolated from the CD24low/– population by positive selection using a biotinylated human CD44 antibody, streptavidin-conjugated magnetic beads, and a MagCellect magnet (Catalog # MAG997, or equivalent). The efficiency of enrichment can be assessed by staining the recovered cells with fluorochrome-conjugated antihuman CD24 and CD44 detection antibodies provided in the kit.
Analysis of CD24low/–CD44+ Cells Isolated using the MagCellect Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit. A small population of CD24low/–CD44+ cells was isolated from MCF-7 human breast adenocarcinoma cells using the MagCellect CD24–CD44+ Breast Cancer Stem Cell Isolation Kit (Catalog # MAGH111). CD24low/–CD44+ cells (upper left quadrant), before (A) and after (B) enrichment, were detected by double-staining with APC-conjugated Mouse Anti-Human CD24 and PE-conjugated Mouse Anti-Human CD44 Detection Antibodies (both provided in the kit). A histogram profiling the enrichment of CD24low/– cells (filled histogram) from the original cell population (open histogram) is shown (C).
Al-Hajj, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3983.