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Memory B Cell Markers

Click on one of the B cell subsets shown in the buttons below to see the human and mouse markers that are commonly used to identify each cell type.

Property title
Human
Human
Cell Surface Markers
Cell Surface Markers
B7-1/CD80+
B7-1/CD80+
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B7-2/CD86+
B7-2/CD86+
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CD10/Neprilysin-
CD10/Neprilysin-
CD19+
CD19+
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CD20/MS4A1+
CD20/MS4A1+
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CD21+
CD21+
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CD23/Fc epsilon RIIlow
CD23/Fc epsilon RIIlow
CD27mid/+
CD27mid/+
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CD38-
CD38-
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CD40+
CD40+
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CD93/C1q R1-
CD93/C1q R1-
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CD95+
CD95+
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DEP-1/CD148+
DEP-1/CD148+
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HLA-DR+
HLA-DR+
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TACI+
TACI+
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Mouse
Mouse
Cell Surface Markers
Cell Surface Markers
5' Nucleotidase/CD73+
5' Nucleotidase/CD73+
B7-1/CD80+
B7-1/CD80+
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B220/CD45Rlow/+
B220/CD45Rlow/+
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CD19+
CD19+
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CD21+
CD21+
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CD27mid/+
CD27mid/+
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CD35-/+
CD35-/+
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CD38-/+
CD38-/+
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CD40+
CD40+
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CD95+
CD95+
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L-Selectin/CD62L-/+
L-Selectin/CD62L-/+
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MHC class II+
MHC class II+
PD-L2/CD273+
PD-L2/CD273+
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Intracellular Markers
Intracellular Markers
OBF1+
OBF1+
Pax5+
Pax5+
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Spi-B+
Spi-B+
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Overview

Memory B cells and plasma cells are two populations of B cells that contribute to immunological memory. Following antigen exposure, a population of naïve B cells differentiates into short-lived plasma cells, and a second population migrates to the B cell follicle. Here, activated naïve B cells seed a germinal center, where they differentiate into memory B cells that can rapidly differentiate into plasma cells after antigen re-exposure. Both human and mouse memory B cells are heterogeneous cell populations. In mice, CD19, CD80, CD73, and PD-L2/CD273 are commonly used to identify memory B cells. Differential expression of these markers suggests that there are at least five distinct mouse memory B cell subsets. In humans, memory B cells are commonly identified by expression of CD27, coupled with low level expression of CD23/Fc epsilon RI, and lack of expression of the plasma cell marker, Syndecan-1/CD138. DEP-1/CD148 is also frequently used to identify human memory B cells, as are high level expression of B7-1/CD80, B7-2/CD86, and CD95. Notably, some populations of CD27- memory B cells in human spleen, tonsil, and blood have also been described, and thus, no marker has been identified that can be used to positively identify all memory B cells. Human memory B cells have historically been defined as IgD- cells, but in the blood and bone marrow, three subsets of circulating memory B cells have been described that are either IgD+IgM+, IgD+IgM-, or IgD-IgM+, also known as IgM-only memory cells.

Data Examples

Detection of Memory B Cells in Human Peripheral Blood Mononuclear Cells by Flow Cytometry. Memory B cells were detected in human peripheral blood mononuclear cells by staining with a Fluorescein-conjugated Mouse Anti-Human CD27/TNFRSF7 Monoclonal Antibody (R&D Systems, Catalog # FAB382F) and an Alexa Fluor® 594-conjugated Mouse Anti-Human CD19 Monoclonal Antibody (R&D Systems, Catalog # FAB4867T).

Detection of Memory B Cells in Mouse Splenocytes. Memory B cells (CD27+/CD19+) from immunized mouse Balb/c splenocytes were detected by staining with an Alexa Fluor® 488-conjugated Rat Anti-Mouse CD27/TNFRSF7 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-11950AF488) and a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966).