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Follicular B Cell Markers

Click on one of the B cell subsets shown in the buttons below to see the human and mouse markers that are commonly used to identify each cell type.

Property title
Human
Human
Cell Surface Markers
Cell Surface Markers
CD10/Neprilysin-
CD10/Neprilysin-
CD19+
CD19+
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CD20/MS4A1+
CD20/MS4A1+
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CD21mid
CD21mid
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CD22/Siglec-2+
CD22/Siglec-2+
CD23/Fc epsilon RII+
CD23/Fc epsilon RII+
CD24low
CD24low
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CD27-
CD27-
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CD38low
CD38low
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CXCR5+
CXCR5+
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HLA-DR+
HLA-DR+
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IgDhigh
IgDhigh
IgMlow
IgMlow
TACI+
TACI+
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Mouse
Mouse
Cell Surface Markers
Cell Surface Markers
B220/CD45R+
B220/CD45R+
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CD1dlow
CD1dlow
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CD19mid
CD19mid
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CD21mid
CD21mid
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CD22/Siglec-2+
CD22/Siglec-2+
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CD23/Fc epsilon RIIhigh
CD23/Fc epsilon RIIhigh
CD24low
CD24low
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CD38+
CD38+
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CD43-
CD43-
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CD93/C1q R1-
CD93/C1q R1-
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CXCR5+
CXCR5+
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IgDhigh
IgDhigh
IgMlow
IgMlow
MHC class II+
MHC class II+
L-Selectin/CD62L+
L-Selectin/CD62L+
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Intracellular Markers
Intracellular Markers
Pax5+
Pax5+
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Overview

Follicular B cells are the main B cell subset in both mice and humans. A small subset of transitional 2 (T2) B cells mature in the bone marrow into follicular B cells, but most immature B cells migrate to the spleen, where they mature into either follicular B cells or marginal zone B cells. Follicular B cell development is driven by strong B cell receptor (BCR) signaling along with blockade of Notch2 signaling. Follicular B cells are freely recirculating cells that home to the lymphoid follicles in the secondary lymphoid organs. Due to their locations adjacent to the T cell zones, these cells are particularly well-suited to participate in T cell-dependent immune responses. Additionally, follicular B cells are found around the bone marrow sinusoids, where they respond to blood-borne pathogens in a T cell-independent manner. Most mouse and human follicular B cells express high levels of IgD and CD23/Fc epsilon RII, and either high or low levels of IgM. In addition, these cells express CD22/Siglec-2, along with low levels of CD1d in mouse, and lower levels of CD21 than what is found on marginal zone B cells in both mouse and human. Following activation, follicular B cells differentiate into short-lived plasma cells in the periphery or enter into T cell-dependent germinal center reactions.

Data Examples

Detection of Follicular and Marginal Zone B-2 Cells in Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966) and an Alexa Fluor 488-conjugated Rat Anti-Mouse CD43 Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43413AF488). CD19+/ CD43 cells were gated. (B) Follicular B-2 cells (CD19mid/CD1dmid/CD23+/CD21low/CD43) and marginal zone B-2 cells (CD19mid/CD1dhigh/CD23/CD21high/CD43) were detected in the CD19+/CD43 population by staining with a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 700-conjugated Rat Anti-Mouse CD1d Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43461AF700). CD1dmid/CD21low and CD1dhigh/CD21high cells were gated. (C) Follicular B-2 cells (CD1dmid/CD21low) and marginal zone B-2 cells (CD1dhigh/CD21high) were stained for CD21 and CD23 using a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 594-conjugated Rat Anti-Mouse CD23/ Fc epsilon RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T).

Detection of IgD and IgM on Follicular and Marginal Zone B-2 Cells from Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with an Alexa Fluor® 488-conjugated Rat Anti-Mouse CD43 Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43413AF488) and a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966). CD19mid/CD43– cells were gated. (B) Follicular B-2 cells (CD19mid/CD1dmid/CD23+/CD21low/CD43) and marginal zone B-2 cells (CD19mid/CD1dhigh/CD23/CD21high/ CD43) were detected in CD19+/CD43 population by staining with a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 594-conjugated Rat Anti-Mouse CD23/Fc epsilon RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T). CD21low/CD23+ and CD21high/CD23 cells were gated. (C) Expression of IgM and IgD on follicular B-2 (CD19mid/ CD23+/CD21low/CD43/IgMlow/IgDhigh) and marginal zone B-2 (CD19mid/CD23/CD21high/CD43/IgMhigh/IgDlow) cells was detected using an Alexa Fluor® 647-conjugated rat anti-mouse IgD monoclonal antibody and a PE-Cy7-conjugated Rat Anti-Mouse IgM Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-42940).