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Marginal Zone B Cell Markers

Click on one of the B cell subsets shown in the buttons below to see the human and mouse markers that are commonly used to identify each cell type.

Property title
Human
Human
Cell Surface Markers
Cell Surface Markers
CD1c/BDCA-1high
CD1c/BDCA-1high
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CD19+
CD19+
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CD20/MS4A1+
CD20/MS4A1+
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CD21high
CD21high
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CD23/Fc epsilon RII-/low
CD23/Fc epsilon RII-/low
CD27+
CD27+
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FCRL3/FcRH3+
FCRL3/FcRH3+
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IgDlow
IgDlow
IgM+
IgM+
TACI+
TACI+
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Mouse
Mouse
Cell Surface Markers
Cell Surface Markers
B220/CD45R+
B220/CD45R+
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CD1dhigh
CD1dhigh
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CD9+
CD9+
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CD19mid
CD19mid
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CD21high
CD21high
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CD22/Siglec-2+
CD22/Siglec-2+
CD23/Fc epsilon RII-/low
CD23/Fc epsilon RII-/low
CD35+
CD35+
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CD43-
CD43-
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CD93/C1q R1-
CD93/C1q R1-
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IgDlow
IgDlow
IgMhigh
IgMhigh
Intracellular Markers
Intracellular Markers
EBF-1+
EBF-1+
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E2A+
E2A+
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Oct2+
Oct2+
Pax5+
Pax5+
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Overview

Marginal zone B cells in mice are located in the outer white pulp of the spleen between the marginal sinus and the red pulp, which allows them to rapidly respond to blood-borne pathogens and participate in both T cell-independent and T cell-dependent immune responses. They develop following migration of transitional 2 (T2) B cells to the spleen, which then mature into either marginal zone B cells or follicular B cells. Marginal zone B cell development is driven by a weak B cell receptor (BCR) signal, along with Notch2 signaling, and BAFF R-mediated canonical NF kappa B signaling. Unlike follicular B cells, marginal zone B cells in mice are immobile, appear to have a long lifespan, and can rapidly mature into plasma cells. In contrast, marginal zone cells in humans are freely recirculating cells that are found in a number of other locations in addition to the spleen. In contrast to follicular B cells, marginal zone B cells express high levels of IgM, and low levels of IgD and CD23/Fc epsilon RII. In addition, both mouse and human marginal zone B cells express high levels of CD1 (CD1d in mice, CD1c in humans), and CD21.

Data Examples

Detection of Marginal Zone and Follicular B-2 Cells in Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966) and an Alexa Fluor 488-conjugated Rat Anti-Mouse CD43 Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43413AF488). CD19+/ CD43 cells were gated. (B) Marginal zone B-2 cells (CD19mid/CD1dhigh/CD23/CD21high/CD43) and follicular B-2 cells (CD19mid/CD1dmid/CD23+/CD21low/CD43) were detected in the CD19+/CD43 population by staining with a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 700-conjugated Rat Anti-Mouse CD1d Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43461AF700). CD1dmid/CD21low and CD1dhigh/CD21high cells were gated. (C) Marginal zone B-2 cells (CD1dhigh/CD21high) and follicular B-2 cells (CD1dmid/CD21low) were stained for CD21 and CD23 using a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 594-conjugated Rat Anti-Mouse CD23/ Fc epsilon RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T).

Detection of IgD and IgM on Marginal Zone and Follicular B-2 Cells from Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with an Alexa Fluor® 488-conjugated Rat Anti-Mouse CD43 Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43413AF488) and a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966). CD19mid/CD43 cells were gated. (B) Marginal zone B-2 cells (CD19mid/CD1dhigh/CD23/CD21high/ CD43) and follicular B-2 cells (CD19mid/CD1dmid/CD23+/CD21low/CD43) were detected in CD19+/CD43 population by staining with a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor® 594-conjugated Rat Anti-Mouse CD23/Fc epsilon RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T). CD21low/CD23+ and CD21high/CD23 cells were gated. (C) Expression of IgM and IgD on marginal zone B-2 (CD19mid/CD23/CD21high/CD43/IgMhigh/IgDlow) and follicular B-2 (CD19mid/ CD23+/CD21low/CD43/IgMlow/IgDhigh) cells was detected using an Alexa Fluor® 647-conjugated rat anti-mouse IgD monoclonal antibody and a PE-Cy7-conjugated Rat Anti-Mouse IgM Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-42940).