0.1% BSA in PBS |
Dissolve 10 mg of BSA in 10 mL of PBS. Sterile filter the solution by syringe filter and store at 2 °C to 8 °C for up to 3 months. |
Endoderm Differentiation Supplement I (500X) |
Reconstitute with 50 μL of sterile 0.1% BSA in PBS. Mix gently. Store at 2 °C to 8 °C for up to 1 month or aliquot and store at ≤ -20 °C in a manual defrost freezer for up to 3 months. Avoid repeated freeze-thaw cycles. |
Endoderm Differentiation Supplement II (500X) |
Reconstitute with 100 μL of sterile 0.1% BSA in PBS. Mix gently. Store at 2 °C to 8 °C for up to 1 month or aliquot and store at ≤ -20 °C in a manual defrost freezer for up to 3 months. Avoid repeated freeze-thaw cycles. |
Ectoderm Differentiation Supplement (500X) |
Reconstitute with 150 μL of sterile 0.1% BSA in PBS. Mix gently. Store at 2 °C to 8 °C for up to 1 month or aliquot and store at ≤ -20 °C in a manual defrost freezer for up to 3 months. Avoid repeated freeze-thaw cycles. |
Mesoderm Differentiation Supplement (500X) |
Reconstitute with 100 μL of sterile 0.1% BSA in PBS. Mix gently. Store at 2 °C to 8 °C for up to 1 month or aliquot and store at ≤ -20 °C in a manual defrost freezer for up to 3 months. Avoid repeated freeze-thaw cycles. |
Differentiation Base Media |
Combine 20 mL of 10X Differentiation Base Media Supplement with 180 mL of RPMI, 2 mL of Penicillin/Streptomycin (optional), and 2 mL of GlutaMAX. Store at 2 °C to 8 °C for up to 2 weeks. |
Endoderm Differentiation Media I |
Dilute Endoderm Differentiation Supplement I stock solution 500-fold in Differentiation Base Media. Prepare fresh as needed. |
Endoderm Differentiation Media II |
Dilute Endoderm Differentiation Supplement II stock solution 500-fold in Differentiation Base Media. Prepare fresh as needed. |
Ectoderm Differentiation Media |
Dilute Ectoderm Differentiation Supplement stock solution 500-fold in Differentiation Base Media. Prepare fresh as needed. |
Mesoderm Differentiation Media |
Dilute Mesoderm Differentiation Supplement stock solution 500-fold in Differentiation Base Media. Prepare fresh as needed. |