Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear
Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear Summary
• Ideal for general cell culture application
• Reduced growth factor formulation provides a more defined culture system
• Polymerizes at 37°C to form a reconstituted basement membrane
• Quality controlled for peformance consistency
Why Use Cultrex RGF BME?
Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. It is processed to reduce matrix-associated growth factors to provide a matrix that can be used to create a more defined model system. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex RGF BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.
Gelling Assay - Cultrex RGF BME gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 14 days at 37 °C.
For research use only. Not for diagnositic use.
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