Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear

Reduced Growth Factor Basement Membrane Extract
Catalog # Availability Size / Price Qty
3433-001-01
3433-005-01
3433-010-01
Assessment of Pluripotency on Cultrex RGF BME.
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Product Details
Citations (26)
FAQs
Reviews (6)

Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear Summary

Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion. It is ideal for cultures that require limited reduced growth factor conditions.

Key Benefits

• Ideal for general cell culture application
• Reduced growth factor formulation provides a more defined culture system
• Polymerizes at 37°C to form a reconstituted basement membrane
• Quality controlled for peformance consistency

Why Use Cultrex RGF BME?

Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. It is processed to reduce matrix-associated growth factors to provide a matrix that can be used to create a more defined model system. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex RGF BME can be used in multiple applications, including maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle, stem cells, and organoid/3-D cell cultures. It can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion, and in vivo tumorigenicity assays.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Specifications

Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
8-12 mg/mL as determined by Lowry assay
Endotoxin Level
≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
Tested following USP <71> sterility guidelines.
Testing Cell Culture
Tube Formation Assay - Cultrex RGF BME supports formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells in a tube formation assay.

Gelling Assay - Cultrex RGF BME gels in less than 30 minutes at 37 °C, and maintains the gelled form in culture medium for a minimum of 14 days at 37 °C.
Viral Testing
Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.
Stability
Product is stable for at least 3 months from the date of receipt when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Species
Mouse

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Bioactivity Assessment of Pluripotency on Cultrex RGF BME. View Larger

Assessment of Pluripotency on Cultrex RGF BME. Alkaline Phosphatase (AP) staining was used to characterize the pluripotency of human induced pluripotent stem (iPS) cells cultured on Cultrex Reduced Growth Factor BME (Catalog # 3433-010-01). Images are shown in 4X and 10X magnification. Data courtesy of GABAeron.

Citations for Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Cancer-associated fibroblasts promote immunosuppression by inducing ROS-generating monocytic MDSCs in lung squamous cell carcinoma
    Authors: H Xiang, CP Ramil, J Hai, C Zhang, H Wang, AA Watkins, R Afshar, P Georgiev, MA Sze, XS Song, PJ Curran, M Cheng, JR Miller, D Sun, A Loboda, Y Jia, LY Moy, A Chi, PE Brandish
    Cancer Immunol Res, 2020;0(0):.  2020
  2. In�Vivo Cell Fate Tracing Provides No Evidence for Mesenchymal to Epithelial Transition in Adult Fallopian Tube and Uterus
    Authors: A Ghosh, SM Syed, M Kumar, TJ Carpenter, JM Teixeira, N Houairia, S Negi, PS Tanwar
    Cell Rep, 2020;31(6):107631.  2020
  3. Endometrial Axin2+ Cells Drive Epithelial Homeostasis, Regeneration, and Cancer following Oncogenic Transformation
    Authors: SM Syed, M Kumar, A Ghosh, F Tomasetig, A Ali, RM Whan, D Alterman, PS Tanwar
    Cell Stem Cell, 2019;26(1):64-80.e13.  2019
  4. mTOR and autophagy pathways are dysregulated in murine and human models of Schaaf-Yang syndrome
    Authors: E Crutcher, R Pal, F Naini, P Zhang, M Laugsch, J Kim, A Bajic, CP Schaaf
    Sci Rep, 2019;9(1):15935.  2019
  5. Vascular-endothelial response to IDH1 mutant fibrosarcoma secretome and metabolite: implications on cancer microenvironment
    Authors: MJ Mao, DE Leonardi
    Am J Cancer Res, 2019;9(1):122-133.  2019
  6. Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization Controls Endochondral Ossification through Hyaluronan Metabolism
    Authors: M Shimoda, H Yoshida, S Mizuno, T Hirozane, K Horiuchi, Y Yoshino, H Hara, Y Kanai, S Inoue, M Ishijima, Y Okada
    Am. J. Pathol, 2017;0(0):.  2017
  7. Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2
    Cancer Res, 2016;0(0):.  2016
  8. Transposon mutagenesis identifies genes that cooperate with mutant Pten in breast cancer progression
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.  2016
  9. The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland.
    Authors: Bittencourt F, Wu S, Bridges J, Miller W
    J Virol, 2014;88(20):11811-24.  2014
  10. Protein kinase D1 mediates anchorage-dependent and -independent growth of tumor cells via the zinc finger transcription factor Snail1.
    Authors: Eiseler T, Kohler C, Nimmagadda S, Jamali A, Funk N, Joodi G, Storz P, Seufferlein T
    J Biol Chem, 2012;287(39):32367-80.  2012
  11. Harvest of superficial layers of fat with a microcannula and isolation of adipose tissue-derived stromal and vascular cells.
    Authors: Trivisonno A, Di Rocco G, Cannistra C, Finocchi V, Torres Farr S, Monti M, Toietta G
    Aesthet Surg J, 0;34(4):601-13.  0
  12. Vesicle-associated membrane protein 2 (VAMP2) but Not VAMP3 mediates cAMP-stimulated trafficking of the renal Na+-K+-2Cl- co-transporter NKCC2 in thick ascending limbs.
    Authors: Caceres P, Mendez M, Ortiz P
    J Biol Chem, 0;289(34):23951-62.  0
  13. PTEN inhibits PREX2-catalyzed activation of RAC1 to restrain tumor cell invasion.
    Authors: Mense S, Barrows D, Hodakoski C, Steinbach N, Schoenfeld D, Su W, Hopkins B, Su T, Fine B, Hibshoosh H, Parsons R
    Sci Signal, 0;8(370):ra32.  0
  14. KLF6 Suppresses Metastasis of Clear Cell Renal Cell Carcinoma via Transcriptional Repression of E2F1.
    Authors: Gao Y, Li H, Ma X, Fan Y, Ni D, Zhang Y, Huang Q, Liu K, Li X, Wang L, Gu L, Yao Y, Ai Q, Du Q, Song E, Zhang X
    Cancer Res, 0;77(2):330-342.  0
  15. GPER mediates activation of HIF1alpha/VEGF signaling by estrogens.
    Authors: De Francesco E, Pellegrino M, Santolla M, Lappano R, Ricchio E, Abonante S, Maggiolini M
    Cancer Res, 0;74(15):4053-64.  0
  16. Activation of CD137 Signaling Promotes Angiogenesis in Atherosclerosis via Modulating Endothelial Smad1/5-NFATc1 Pathway.
    Authors: Weng J, Wang C, Zhong W, Li B, Wang Z, Shao C, Chen Y, Yan J
    J Am Heart Assoc, 0;6(3):.  0
  17. Effects of ulipristal acetate on human embryo attachment and endometrial cell gene expression in an in vitro co-culture system.
    Authors: Berger C, Boggavarapu N, Menezes J, Lalitkumar P, Gemzell-Danielsson K
    Hum Reprod, 0;30(4):800-11.  0
  18. Isogenic blood-brain barrier models based on patient-derived stem cells display inter-individual differences in cell maturation and functionality.
    Authors: Patel R, Page S, Al-Ahmad A
    J Neurochem, 0;142(1):74-88.  0
  19. The hippo pathway effector YAP regulates motility, invasion, and castration-resistant growth of prostate cancer cells.
    Authors: Zhang L, Yang S, Chen X, Stauffer S, Yu F, Lele S, Fu K, Datta K, Palermo N, Chen Y, Dong J
    Mol Cell Biol, 0;35(8):1350-62.  0
  20. Function of carbonic anhydrase IX in glioblastoma multiforme.
    Authors: Proescholdt M, Merrill M, Stoerr E, Lohmeier A, Pohl F, Brawanski A
    Neuro Oncol, 0;14(11):1357-66.  0
  21. PI3K/mTOR dual inhibitor VS-5584 preferentially targets cancer stem cells.
    Authors: Kolev V, Wright Q, Vidal C, Ring J, Shapiro I, Ricono J, Weaver D, Padval M, Pachter J, Xu Q
    Cancer Res, 0;75(2):446-55.  0
  22. Preclinical Antitumor Efficacy of BAY 1129980-a Novel Auristatin-Based Anti-C4.4A (LYPD3) Antibody-Drug Conjugate for the Treatment of Non-Small Cell Lung Cancer.
    Authors: Willuda J, Linden L, Lerchen H, Kopitz C, Stelte-Ludwig B, Pena C, Lange C, Golfier S, Kneip C, Carrigan P, McLean K, Schuhmacher J, von Ahsen O, Muller J, Dittmer F, Beier R, El Sheikh S, Tebbe J, Leder G, Apeler H, Jautelat R, Ziegelbauer K, Kreft B
    Mol Cancer Ther, 0;16(5):893-904.  0
  23. CLM94, a novel cyclic amide with anti-VEGFR-2 and antiangiogenic properties, is active against primary anaplastic thyroid cancer in vitro and in vivo.
    Authors: Antonelli A, Bocci G, La Motta C, Ferrari S, Fallahi P, Ruffilli I, Di Domenicantonio A, Fioravanti A, Sartini S, Minuto M, Piaggi S, Corti A, Ali G, Di Desidero T, Berti P, Fontanini G, Danesi R, Da Settimo F, Miccoli P
    J Clin Endocrinol Metab, 0;97(4):E528-36.  0
  24. Combination of an allosteric Akt Inhibitor MK-2206 with etoposide or rapamycin enhances the antitumor growth effect in neuroblastoma.
    Authors: Li Z, Yan S, Attayan N, Ramalingam S, Thiele C
    Clin Cancer Res, 0;18(13):3603-15.  0
  25. Conditioned medium from human amniotic mesenchymal stromal cells limits infarct size and enhances angiogenesis.
    Authors: Danieli P, Malpasso G, Ciuffreda M, Cervio E, Calvillo L, Copes F, Pisano F, Mura M, Kleijn L, de Boer R, Viarengo G, Rosti V, Spinillo A, Roccio M, Gnecchi M
    Stem Cells Transl Med, 0;4(5):448-58.  0
  26. Metformin Reduces Prostate Tumor Growth, in a Diet-Dependent Manner, by Modulating Multiple Signaling Pathways.
    Authors: Sarmento-Cabral A, L-Lopez F, Gahete M, Castano J, Luque R
    Mol Cancer Res, 0;15(7):862-874.  0

FAQs

  1. What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    • Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  2. How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  3. How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    • Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

  4. What is the Tube Formation Assay?

    • The Tube Formation Assay is based on the ability of endothelial cells to form three-dimensional capillary-like tubular structures when cultured on a hydrogel of reconstituted basement membrane, such as Cultrex Basement Membrane Extract (BME).

  5. What are the advantages of the Tube Formation Assay?

    • The Tube Formation Assay is the most widely used in vitro angiogenesis assay. The assay is rapid, inexpensive and quantifiable. It can be used to identify potentially angiogenic and anti-angiogenic factors, to determine endothelial cell phenotype, and to study pathways and mechanisms involved in angiogenesis. It can be performed in a high throughput mode to screen for a large number of compounds.

  6. What cell types can be used in the Tube Formation Assay?

    • The Tube Formation Assay is specific for endothelial cells, either primary cells or immortalized cell lines. Only endothelial cells form capillary-like structures with a lumen inside. Other endothelial cell types form other structures.

  7. What are the variables associated with the Tube Formation Assay?

    • The major variables associated with tube formation are composition of the Cultrex Basement Membrane Extract (BME) hydrogel, thickness of the hydrogel, cell density, composition of angiogenic factors in the assay medium, and assay period.

  8. Which Cultrex Basement Membrane Extract (BME) should I use for the Tube Formation Assay?

    • Cultrex Reduced Growth Factor BME (RGF BME) is generally used for testing compounds that promote angiogenesis because formation of capillary-like structures (tubes) is significantly less compared to non-growth factor reduced varieties of Cultrex BME. The Cultrex In Vitro Angiogeneis Assay (Tube Formation) includes a qualified production lot of Cultrex RGF BME that exhibits reduced background tube formation in the absence of angiogenic factors.

  9. How do I reduce spontaneous formation of tubular structures on Cultrex BME in the absence of angiogenic factors?

    • Primary endothelial cells, such as Human Umbilical Vein Endothelial Cells (HUVECs) form capillary-like structures in the absence of added angiogenic factors less often than immortalized endothelial cells. Generally, reducing the number of cells per cm2 plated onto Cultrex BME will result in less background or spontaneous tube formation. Titrate the number of cells and find optimal conditions for your specific cell line. When endothelial cells fully form capillary structures in response to angiogenic activators, but not in their absence, you may proceed.

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Reviews for Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear

Average Rating: 4.8 (Based on 6 Reviews)

5 Star
83.33%
4 Star
16.67%
3 Star
0%
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1 Star
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Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear
By Anonymous on 12/02/2020
Application:

Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear
By Anonymous on 03/09/2020
Application:

Cultrex RGF Basement Membrane Extract
By Anonymous on 01/13/2020
Application: In vivo study

Cultrex RGF Basement Membrane Extract
By Anonymous on 05/27/2019
Application: In vivo study
Reason for Rating: I used Cultrex for an xenograft tumor growth Invivo study. I mixed Breast Cancer cells and cultrex in an 1:1 ratio and injected into mice and followed tumor growth. Cultrex helped to hold the cells together and which helped with the tumor growth.

Cultrex RGF Basement Membrane Extract
By Anonymous on 05/27/2019
Application: In vivo study

Cultrex RGF Basement Membrane Extract
By Anonymous on 02/08/2019
Application: Stem cell culture and maintenance