Cpf1 Proteins and Enzymes

Clustered regularly interspaced short palindromic repeats (CRISPRs) are derived from DNA fragments of bacteriophages that infect prokaryotes. When infected, the bacteria capture snips of DNA from the invading virus generating CRISPR arrays. During subsequent infections, the bacteria produce RNA segments from the CRISPR arrays to target the virus' DNA. CRISPR-associated protein 9 (Cas9) from Streptococcus pyogenes is a class 2/type II RNA-guided, DNA binding, and DNA cleaving enzyme. Cas9 ...