MauriceFlex System
Automated icIEF, CE‑SDS, and Charge Variant Fractionation
Automated icIEF, icIEF Fractionation, and CE-SDS on One System
MauriceFlex™ system is an automated capillary electrophoresis system that combines icIEF, icIEF‑based fractionation, and CE‑SDS on a single platform. In addition to routine charge and size analysis, MauriceFlex enables collection of charge variants for in-depth characterization using a variety of downstream methods, such as intact mass spectrometry, reduced-MS, peptide mapping, binding analysis with SPR, aggregate analysis with mass photometry and other orthogonal analytical techniques.
How can MauriceFlex System Help you?
Simplify Charge Variant Analysis
- icIEF fractionation enables separation and collection of charge variants on the same system, eliminating the need for methods like ion-exchange chromatography (IEX)
- Fractions are collected in ammonium acetate and do not contain urea, and can thus be analyzed with mass spectrometry without additional sample preparation
- The collected fractions can be characterized by various analytical instruments without needing buffer exchange
Biomolecular and Protein Fractionation on MauriceFlex
Get a deeper characterization of charge variants with fractionation
- An applied electric field creates a pH gradient across the capillary inside the MauriceFlex cartridge
- Proteins separate by migrating to different pH values on the gradient, based on their individual isoelectric point (pI)
- Introduction of ammonium acetate chargers the pH gradient, mobilizing these separated proteins, and results in elution (fractionation)
MauriceFlex Specifications for icIEF, and cIEF Fractionation
| Feature | cIEF | cIEF 400 | cIEF Fractionation |
| Minimum Sample Volume | 50 µL | 50 µL | 100 µL |
| Sample Delivery | Vacuum | Vacuum | Vacuum |
| Typical Separation Time | 6–10 min (molecule-dependent) | 6–10 min (molecule-dependent) | 40 – 50 min (molecule-dependent) |
| Detection Capability | UV Absorbance at 280 nm Fluorescence: Ex 280 nm, Em 320– 450 nm | UV Absorbance at 280 nm Fluorescence: Ex 280 nm, Em 320– 450 nm | Fluorescence: Ex 280 nm, Em 320-450nm |
| Typical Voltage | Pre-focusing: 1500 V, focusing: 3000 V | Pre-focusing: 1500 V, focusing: 3000 V | Pre-focusing: 500 V and 1000 V Focusing: 1500 V |
| Sample Injections per Cartridge | 200 maximum (max 25 batches) | 400 maximum (max 40 batches) | Maximum 15 injections |
| Maximum Sample Injections per Batch | 100 | 100 | 1 (fractionation) 4 (cIEF) |
| pI/Size Range | 2.85–10.45 | 2.85–10.45 | 3-10 |
| pI/Sizing CV | 1% | 1% | 1% |
| CV for Peaks >10% Composition | ≤5% (Intra-batch), ≤6% (Inter-batch) | ≤5% (Intra-batch), ≤6% (Inter-batch) | ≤10% (Inter-batch) |
| pI/Sizing Resolution | 0.05 pI units (for wide range 3–10 ampholytes) | 0.05 pI units (for wide range 3–10 ampholytes) | |
| Dynamic Range | 2 logs | 2 logs | |
| Linearity | >0.995 | >0.995 | |
| Sensitivity (LOD) | 0.7 µg/mL (Native fluorescence) 3.0 µg / mL(Absorbance) (Values based on a monoclonal antibody) | 0.7 µg/mL (Native fluorescence) 3.0 µg / mL(Absorbance) (Values based on a monoclonal antibody) |
MauriceFlex Specifications for CE-SDS
| Feature | CE-SDS PLUS | Turbo CE-SDS |
| Minimum Sample Volume | 50 µL | 100 µL |
| Sample Delivery | Electrokinetic | Electrokinetic |
| Typical Separation Time | Reduced IgG: 25 min, Non-reduced IgG: 35 min | Reduced IgG: 5.5 min Non-reduced IgG: 8 min |
| Detection Capability | UV Absorbance at 220 nm | UV Absorbance at 220 nm |
| Typical Voltage | Separation: 5750 V | Separation: 4200 V |
| Sample Injections per Cartridge | 500 maximum (max 25 batches) | max 25 batches |
| Maximum Sample Injections per Batch | 48 | 96 |
| pI/Size Range | 10–270 kDa | 10–270 kDa |
| pI/Sizing CV | ≤2% | <2% |
| CV for Peaks >10% Composition | N/A | N/A |
| Relative Migration Time CV | <1% for reduced IgG | <5% |
| pI/Sizing Resolution | ≥1.5 for NGHC/HC IgG Standard | ≥1.0 for NGHC/HC IgG Standard |
| Dynamic Range | 2 logs | 2 logs |
| Linearity | >0.995 | >0.995 |
| Sensitivity (LOD) | 0.3 µg/mL (Value based on Internal Standard) | 0.6 µg/mL (Value based on Internal Standard) |
| Sample Tray Options | 96-well plates or 48 vials (cIEF Fractionation: 96-well plates only) |
| Power | 100 V–240 V (AC), 50/60 Hz, 500 W |
| Voltage Range | 0–6,500 V |
| Temperature Control Range | 4-25 °C (cIEF Fractionation: 10-25 °C) |
| Dimensions | 44cm H x 42cm W x 61cm D |
| Weight | 46 kg (100 lb) |
Compatible Products
| Product | Catalog Number |
| MauriceFlex System | 090-158 |
| CE-SDS PLUS Cartridge | PS-MC02-SP |
| Turbo CE-SDS Cartridge | PS-MC02-TS |
| CE-SDS PLUS Application Kit | PS-MAK03-S |
| Turbo CE-SDS Application Kit | PS-MAK01-TS |
| cIEF Cartridge | PS-MC02-C |
| icIEF 400 Cartridge | PS-MC02-400C |
| cIEF Method Development Kit | PS-MDK01-C |
| cIEF Fractionation Application Kit | PS-MAK01-F |
| cIEF Fractionation Cartridge | PS-MC02-F |
Read this interview with Dr. Jana Stockel ad Dr. David Richards from MilliporeSigma, who briefly describe how the company is paving the way for ADC research and the modern techniques they use to accelerate workflows.
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