14-3-3 gamma Antibody (KC21) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-81016

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # KC21

Format

Azide and BSA Free
View all 14-3-3 gamma Primary Antibodies »

Product Specifications

Immunogen

N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated [UniProt# P61981]

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 gamma Antibody (KC21) - Azide and BSA Free

Western Blot: 14-3-3 gamma Antibody (KC21)Azide and BSA Free [NBP2-81016]

Western Blot: 14-3-3 gamma Antibody (KC21)Azide and BSA Free [NBP2-81016]

Western Blot: 14-3-3 gamma Antibody (KC21) - Azide and BSA Free [NBP2-81016] - 14-3-3 gamma detected in lysates using NB100-406. Lane 1: HeLa cell lysates, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: 14-3-3 gamma Antibody (KC21) - Azide and BSA Free [NBP2-81016]

Immunocytochemistry/ Immunofluorescence: 14-3-3 gamma Antibody (KC21) - Azide and BSA Free [NBP2-81016]

Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (KC21) - Azide and BSA Free [NBP2-81016] - 14-3-3 gamma [ac Val2] antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.
Western Blot: 14-3-3 gamma Antibody (KC21)Azide and BSA Free [NBP2-81016]

Western Blot: 14-3-3 gamma Antibody (KC21)Azide and BSA Free [NBP2-81016]

Western Blot: 14-3-3 gamma Antibody (KC21) - Azide and BSA Free [NBP2-81016] - (1) HeLa, (2) PC12 and (3) NIH-3T3 whole cell lysates were separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with Anti-14-3-3 gamma [Ac Val2] antibody at 2 ug/ml for 1 hour, followed by incubation a 1:10,000 dilution of anti-rabbit HRP. The protein was detected at approximately 28 kDa after incubation with SuperSignal West Pico Chemiluminescent substrate. Image from the standard format of this antibody.
Simple Western: 14-3-3 gamma Antibody (KC21)Azide and BSA Free [NBP2-81016]

Simple Western: 14-3-3 gamma Antibody (KC21)Azide and BSA Free [NBP2-81016]

Simple Western: 14-3-3 gamma Antibody (KC21) - Azide and BSA Free [NBP2-81016] - Lane view shows a specific band for 14-3-3 Gamma in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. Image from the standard format of this antibody.

Applications for 14-3-3 gamma Antibody (KC21) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Simple Western

1:12.5

Western Blot

1:1000-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 gamma

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities.

Alternate Names

1433 gamma, YWHAG

Gene Symbol

YWHAG

Additional 14-3-3 gamma Products

Product Documents for 14-3-3 gamma Antibody (KC21) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for 14-3-3 gamma Antibody (KC21) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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