5T4 Antibody (24GB2035)

Novus Biologicals | Catalog # NBP3-51588

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB2035
View all 5T4 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human 5T4 (Uniprot: Q13641)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5T4 Antibody (24GB2035)

5T4 Antibody (24GB2035)

Flow Cytometry: 5T4 Antibody (24GB2035)[NBP3-51588] -

Validation of 5T4 knockdown using flow cytometry. Wild-type(WT, Blue) and 5T4 knockdown(KD, Green) HT-1080 cells were stained with 5T4 antibody(1:2,000) and analyzed using BD flow cytometer.
5T4 Antibody (24GB2035)

Flow Cytometry: 5T4 Antibody (24GB2035)[NBP3-51588] -

Flow cytometric analysis of 5T4expression in HAP-1 cells using 5T4 antibody (1:2,000). Green, isotype control; red, Trophoblast glycoprotein.
5T4 Antibody (24GB2035)

Western Blot: 5T4 Antibody (24GB2035)[NBP3-51588] -

Western blotting analysis using 5T4 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for 5T4 Antibody (24GB2035)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5T4

The 5T4 gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is ass

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Gene Symbol

TPBG

Additional 5T4 Products

Product Documents for 5T4 Antibody (24GB2035)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 5T4 Antibody (24GB2035)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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