Abhd5 Antibody (JE60-62)

Flow Cytometry: Abhd5 Antibody (JE60-62) [NBP3-31989]

Flow Cytometry: Abhd5 Antibody (JE60-62) [NBP3-31989] - Flow cytometric analysis of NIH/3T3 cells labeling Abhd5.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31989, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: Abhd5 Antibody (JE60-62) [NBP3-31989]

Western Blot: Abhd5 Antibody (JE60-62) [NBP3-31989] - Western blot analysis of Abhd5 on different lysates with Rabbit anti-Abhd5 antibody (NBP3-31989) at 1/2,000 dilution.

Lane 1: NIH/3T3 cell lysate (20 ug/Lane)
Lane 2: Mouse pancreas tissue lysate (40 ug/Lane)
Lane 3: Mouse kidney tissue lysate (40 ug/Lane)

Predicted band size: 39 kDa
Observed band size: 43 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31989) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.