Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-09003

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Block/Neutralize, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # Ig2838

Format

Azide and BSA Free
View all Adenosine A2aR Primary Antibodies »

Product Specifications

Immunogen

Human recombinant A2-adenosine receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free [NBP3-09003]

Immunocytochemistry/ Immunofluorescence: Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free [NBP3-09003]

Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (Ig2838) - Chimeric [NBP3-09003] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Ig2838 (NBP3-09003) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing granular cytoplasmic staining, possibly of protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09003, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.

Applications for Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

This antibody binds to the A2 adenosine receptor, a G-protein-coupled receptor for adenosine that, upon ligand binding, activates adenylyl cyclase. The antibody acts as an allosteric inhibitor.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Adenosine A2aR

Adenosine A2a Receptor encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Long Name

Adenosine A2A Receptor

Alternate Names

A2aR, Adenosine A2a R, ADORA2A, RDC8

Gene Symbol

ADORA2A

Additional Adenosine A2aR Products

Product Documents for Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Adenosine A2aR Antibody (Ig2838) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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