Recombinant Rat Adiponectin/Acrp30 FLAG Protein

Novus Biologicals | Catalog # NBP3-18139

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Key Product Details

Source

HEK293

Accession Number

Q8K3R4

Tag

FLAG

Applications

Western Blot, Bioactivity
View all Adiponectin/Acrp30 Proteins and Enzymes »

Product Specifications

Description

Rat adiponectin (aa 16-244) fused at the N-terminus to a FLAG(R)-tag.

Source: HEK 293 cells Uniprot ID: Q8K3R4

TMW: ~30kDa (SDS-PAGE); ~180kDa (Hexamer, by SEC)

Purity

>95% pure by SDS-PAGE

Endotoxin Level

<0.01EU/ug purified protein (LAL test).

Activity

Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat Adiponectin/Acrp30 FLAG Protein

Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139]

Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139]

Western Blot: Recombinant Rat Adiponectin/Acrp30 FLAG Protein [NBP3-18139] - Effect of Adiponectin/Acrp30 (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.Method: C2C12 myocytes were treated with 25ug/ml for 15min after serum starvation for 24hr. 100ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.1: 25ug/ml of Adiponectin/Acrp30 (rat) (rec.).2: 25ug/ml of a FLAG(R)-tagged control protein.3: phosphate-buffered saline.

Formulation, Preparation, and Storage

NBP3-18139
Formulation Lyophilized from 30mM Tris-Cl, pH 8.0 + 100mM NaCl
Preservative No Preservative
Concentration Lyoph
Reconstitution Reconstitute with 50 ul endotoxin-free water (1mg/ml after reconstitution)
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adiponectin/Acrp30

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Gene Symbol

ADIPOQ

UniProt

Q8K3R4 (Rat)

Additional Adiponectin/Acrp30 Products

Product Documents for Recombinant Rat Adiponectin/Acrp30 FLAG Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Rat Adiponectin/Acrp30 FLAG Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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