African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25366
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Lateral Flow Assay
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1218 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of African Swine Fever Virus p54. The exact sequence is proprietary.
Reactivity Notes
African Swine Fever Virus
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] -
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-13672 [GT1075]) Detection: ASFV p54 antibody (NBP3-25366 [HL1218]) Samples (40 ng) : 1. ASFV p54 (ECD) protein 2. Lysis bufferLateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] -
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25369 [HL1287]) Detection: ASFV p54 antibody (NBP3-25366 [HL1218]) Samples (10 ng) : 1. ASFV p54 (ECD) protein 2. Lysis bufferLateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] -
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-13671 [GT853]) Detection: ASFV p54 antibody (NBP3-25366 [HL1218]) Samples (40 ng) : 1. ASFV p54 (ECD) protein 2. Lysis bufferLateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] -
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25368 [HL1286]) Detection: ASFV p54 antibody (NBP3-25366 [HL1218]) Samples (80 ng) : 1. ASFV p54 (ECD) protein 2. Lysis bufferELISA: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] -
ELISA: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] - Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below. Capture: ASFV p54 antibody [HL1218] (NBP3-25366) (5 ug/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1219] (NBP3-25367) (0.1 ug/mL) Please notice that NBP3-25367 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25366. Please contact us for custom HRP-conjugated antibody.Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] -
Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] - ASFV p54 antibody [HL1218] detects ASFV p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASFV p54 stained by ASFV p54 antibody [HL1218] (NBP3-25366) diluted at 1:500. Blue: Fluoroshield with DAPI.Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] -
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] - Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair. Capture: ASFV p54 antibody (NBP3-25367 [HL1219]) Detection: ASFV p54 antibody (NBP3-25366 [HL1218]) Samples (20 ng) : 1. ASFV p54 (ECD) protein 2. Lysis bufferWestern Blot: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] -
Western Blot: African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free [NBP3-25366] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1218] (NBP3-25366) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:5000-1:500000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: African Swine Fever Virus p54
Alternate Names
ASFV p54, Ba71V-126, E183L, Envelope protein p54, Structural protein p54
Gene Symbol
E183L
Additional African Swine Fever Virus p54 Products
Product Documents for African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for African Swine Fever Virus p54 Antibody (HL1218) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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