Antennapedia Control Peptide

Immunocytochemistry/ Immunofluorescence: Antennapedia Control Peptide [NBP2-29334]

Immunocytochemistry/Immunofluorescence: Antennapedia Control Peptide [NBP2-29334] - NBD inhibitory peptide but not Control peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were treated with 100 uM of Control (A & B) or NBD peptide (C & D) for 12 hr, cytospun, plated on glass slides, air dried for 1 hr at room temperature and fixed with cold acetone. Slides were blocked with 5% normal goat serum for 1 hr and then incubated with rabbit polyclonal anti-human p65 antibody (A & C) followed by Ig-Alexa 594 second step. In control peptide treated cells, p65 translocates to nucleus (A), whereas NBD peptide prevents translocation of p65 into the nucleus (C). B & D: Nuclear staining with DNA binding dye. (Courtesy of Dr. B.B. Aggarwal, MD Anderson Cancer Center, Houston, TX)