ARA54 Antibody (4G9) - Azide and BSA Free

Novus Biologicals | Catalog # H00009604-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G9

Format

Azide and BSA Free
View all ARA54 Primary Antibodies »

Product Specifications

Immunogen

RNF14 (NP_004281, 217 a.a. ~ 316 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LFLCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATPGQVKELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQL

Reactivity Notes

Human. Other species not tested.

Specificity

RNF14 - ring finger protein 14

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ARA54 Antibody (4G9) - Azide and BSA Free

Western Blot: ARA54 Antibody (4G9) [H00009604-M01]

Western Blot: ARA54 Antibody (4G9) [H00009604-M01]

Western Blot: ARA54 Antibody (4G9) [H00009604-M01] - Analysis of RNF14 expression in transfected 293T cell line by RNF14 monoclonal antibody (M01), clone 4G9.Lane 1: RNF14 transfected lysate(39.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ARA54 Antibody (4G9) [H00009604-M01]

Immunocytochemistry/ Immunofluorescence: ARA54 Antibody (4G9) [H00009604-M01]

Immunocytochemistry/Immunofluorescence: ARA54 Antibody (4G9) [H00009604-M01] - Analysis of monoclonal antibody to RNF14 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: ARA54 Antibody (4G9) [H00009604-M01]

Western Blot: ARA54 Antibody (4G9) [H00009604-M01]

Western Blot: ARA54 Antibody (4G9) [H00009604-M01] - Analysis of RNF14 over-expressed 293 cell line, cotransfected with RNF14 Validated Chimera RNAi ( Cat # H00009604-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF14 monoclonal antibody (M01), clone 4G9 (Cat # H00009604-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: ARA54 Antibody (4G9) [H00009604-M01] - Detection limit for recombinant GST tagged RNF14 is approximately 0.1ng/ml as a capture antibody.

Applications for ARA54 Antibody (4G9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA and immunofluorescence.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARA54

The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Long Name

Androgen Receptor Associated Protein 54

Alternate Names

HFB30, RNF14, TRIAD2

Entrez Gene IDs

9604 (Human)

Gene Symbol

RNF14

OMIM

605675 (Human)

UniProt

NP_004281 (Human)

Additional ARA54 Products

Product Documents for ARA54 Antibody (4G9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ARA54 Antibody (4G9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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