ARF5 Antibody (1B4) - Azide and BSA Free

Novus Biologicals | Catalog # H00000381-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bacteria, Canine

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B4

Format

Azide and BSA Free
View all ARF5 Primary Antibodies »

Product Specifications

Immunogen

ARF5 (AAH03043, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

ARF5 - ADP-ribosylation factor 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ARF5 Antibody (1B4) - Azide and BSA Free

Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - Analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M01), clone 1B4.Lane 1: ARF5 transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ARF5 Antibody (1B4) [H00000381-M01]

Immunocytochemistry/ Immunofluorescence: ARF5 Antibody (1B4) [H00000381-M01]

Immunocytochemistry/Immunofluorescence: ARF5 Antibody (1B4) [H00000381-M01] - Analysis of monoclonal antibody to ARF5 on HeLa cell. Antibody concentration 15 ug/ml.
Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - ARF5 monoclonal antibody (M01), clone 1B4. Analysis of ARF5 expression in HeLa.
Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - ARF5 monoclonal antibody (M01), clone 1B4. Analysis of ARF5 expression in PC-12.
Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - ARF5 monoclonal antibody (M01), clone 1B4. Analysis of ARF5 expression in Raw 264.7.
Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01]

Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - ARF5 monoclonal antibody (M01), clone 1B4 Analysis of ARF5 expression in A-431.
ELISA: ARF5 Antibody (1B4) [H00000381-M01]

ELISA: ARF5 Antibody (1B4) [H00000381-M01]

ELISA: ARF5 Antibody (1B4) [H00000381-M01] - Detection limit for recombinant GST tagged ARF5 is approximately 0.3ng/ml as a capture antibody.

Applications for ARF5 Antibody (1B4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARF5

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.

Alternate Names

ADP-ribosylation factor 5

Entrez Gene IDs

381 (Human)

Gene Symbol

ARF5

OMIM

103188 (Human)

UniProt

AAH03043 (Human)

Additional ARF5 Products

Product Documents for ARF5 Antibody (1B4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ARF5 Antibody (1B4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ARF5 Antibody (1B4) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ARF5 Antibody (1B4) - Azide and BSA Free

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  • Q: I am looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each protein that won't cross react with the other protein?

    A: I checked the sequence similarity between ARF4 and ARF5, and there is very high homology between the two proteins. We have not specifically tested our antibodies for cross-reactivity between the two, but I think it's likely that they will.

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