ATG9B Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-13108
![Western Blot: ATG9B Antibody [NBP3-13108]](https://resources.rndsystems.com/images/products/ATG9B-Antibody-Western-Blot-NBP3-13108-img0001.jpg "Western Blot: ATG9B Antibody [NBP3-13108]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG9B. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for ATG9B Antibody - BSA Free
Western Blot: ATG9B Antibody [NBP3-13108]
Western Blot: ATG9B Antibody [NBP3-13108] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9B antibody (NBP3-13108) diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody [NBP3-13108]](https://resources.rndsystems.com/images/products/ATG9B-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13108-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ATG9B Antibody [NBP3-13108]")
Immunocytochemistry/ Immunofluorescence: ATG9B Antibody [NBP3-13108]
Immunocytochemistry/Immunofluorescence: ATG9B Antibody [NBP3-13108] - ATG9B antibody detects ATG9B protein at cytoplasm by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 500 uM Chloroquine for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG9B protein stained by ATG9B antibody (NBP3-13108) diluted at 1:500. Blue: Hoechst 33342 staining.Applications for ATG9B Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATG9B
Alternate Names
APG9L2APG9-like 2, ATG9 autophagy related 9 homolog B (S. cerevisiae), autophagy-related protein 9B, endothelial nitric oxide synthase antisense, nitric oxide synthase 3 antisense, Nitric oxide synthase 3-overlapping antisense gene protein, NOS3ASautophagy 9-like 2 protein, Protein sONE, SONEFLJ14885
Gene Symbol
ATG9B
Additional ATG9B Products
Product Documents for ATG9B Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ATG9B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for ATG9B Antibody - BSA Free
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Q: How much is the molecular weight of ATG9B protein?
A: The predicted molecular weight of ATG9B protein is 101kDa
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