BDNF Antibody (3D9C5) - BSA Free

Novus Biologicals | Catalog # NBP2-37276

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D9C5

Format

BSA Free
View all BDNF Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BDNF (AA: 19-248) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse BDNF Antibody (3D9C5) - BSA Free (NBP2-37276) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BDNF Antibody (3D9C5) - BSA Free

Western Blot: BDNF Antibody (3D9C5)BSA Free [NBP2-37276]

Western Blot: BDNF Antibody (3D9C5)BSA Free [NBP2-37276]

Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.
Immunocytochemistry/ Immunofluorescence: BDNF Antibody (3D9C5) - BSA Free [NBP2-37276]

Immunocytochemistry/ Immunofluorescence: BDNF Antibody (3D9C5) - BSA Free [NBP2-37276]

Immunocytochemistry/Immunofluorescence: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Western Blot: BDNF Antibody (3D9C5)BSA Free [NBP2-37276]

Western Blot: BDNF Antibody (3D9C5)BSA Free [NBP2-37276]

Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)
Western Blot: BDNF Antibody (3D9C5)BSA Free [NBP2-37276]

Western Blot: BDNF Antibody (3D9C5)BSA Free [NBP2-37276]

Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: BDNF Antibody (3D9C5) - BSA Free [NBP2-37276]

ELISA: BDNF Antibody (3D9C5) - BSA Free [NBP2-37276]

ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for BDNF Antibody (3D9C5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BDNF

FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family. BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Gene Symbol

BDNF

Additional BDNF Products

Product Documents for BDNF Antibody (3D9C5) - BSA Free

Certificate of Analysis

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Product Specific Notices for BDNF Antibody (3D9C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BDNF Antibody (3D9C5) - BSA Free

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Protocols

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FAQs

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