BMI-1 Antibody (23GB685)

Novus Biologicals | Catalog # NBP3-52016

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB685
View all BMI-1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human BMI-1 (Uniprot: P35226)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BMI-1 Antibody (23GB685)

BMI-1 Antibody (23GB685)

Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody (23GB685)[NBP3-52016] -

Immunocytochemical staining of HeLa cells with BMI-1 antibody (1:1,000). Nuclei were stained blue with DAPI; BMI-1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
BMI-1 Antibody (23GB685)

Western Blot: BMI-1 Antibody (23GB685)[NBP3-52016] -

Western blotting analysis using BMI-1 antibody. BMI-1expression in wild type (WT) and BMI-1shRNA knockdown (KD) 293T cells with 30 ug of total cell lysates. GAPDH serves as a loading control. The blot was incubated with BMI-1 antibody(1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (1:50,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for BMI-1 Antibody (23GB685)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BMI-1

Bmi1 polycomb ring finger oncogene regulates the cell cycle inhibitor genes p16 and p19, with implications in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi 1 has been shown to induce telomerase activity and immortalize human mammary epithelial cells, and extend the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi-1 is also required for efficient self-renewing cell division in adult hematopoietic and neural stem cells.

Aberrant expression of Bmi-1 has been found in several types of cancer in the bladder, skin, blood, prostate, breast, ovaries, andcolon. Its overexpression is especially pronounced in mantle cell lymphomas. Thereforem Bmi1 antibodies will be useful tools for many cancer development studies.

Long Name

B Lymphoma Mo-MLV Insertion Region 1

Alternate Names

BMI1, PCGF4, RNF51

Gene Symbol

BMI1

Additional BMI-1 Products

Product Documents for BMI-1 Antibody (23GB685)

Certificate of Analysis

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Product Specific Notices for BMI-1 Antibody (23GB685)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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