Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 849203
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Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD277/BTN3A1
Met1-Gly254
Accession # O00481

Specificity

Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for BTN3A1/2/3 Antibody (849203) [DyLight 350]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: BTN3A1/2/3

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]

Long Name

Butyrophilin, Subfamily 3, Member A1/2/3

Alternate Names

BT3.1, BTF5, BTN3.1, butyrophilin subfamily 3 member A1, CD277, dJ45P21.3 (butyrophilin, subfamily 3, member A1)

Gene Symbol

BTN3A1

Additional BTN3A1/2/3 Products

Product Documents for BTN3A1/2/3 Antibody (849203) [DyLight 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BTN3A1/2/3 Antibody (849203) [DyLight 350]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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