C4 binding protein B Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38250

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all C4 binding protein B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 17-251 of human C4 binding protein B (NP_001017364.1).

Sequence:
SDEHCPELPPVDNSIFVAKEVEGQILGTYVCIKGYHLVGKKTLFCNASKEWDNTTTECRLGHCPDPVLVNGEFSSSGPVNVSDKITFMCNDHYILKGSNRSQCLEDHTWAPPFPICKSRDCDPPGNPVHGYFEGNNFTLGSTISYYCEDRYYLVGVQEQQCVDGEWSSALPVCKLIQEAPKPECEKALLAFQESKNLCEAMENFMQQLKESGMTMEELKYSLELKKAELKAKLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for C4 binding protein B Antibody - BSA Free

C4 binding protein B Antibody

Western Blot: C4 binding protein B Antibody [NBP3-38250] -

Western Blot: C4 binding protein B Antibody [NBP3-38250] - Western blot analysis of various lysates, using C4 binding protein B Rabbit pAb at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
C4 binding protein B Antibody

Immunohistochemistry: C4 binding protein B Antibody [NBP3-38250] -

Immunohistochemistry: C4 binding protein B Antibody [NBP3-38250] - Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat liver using C4BPB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for C4 binding protein B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: C4 binding protein B

C4BPB( AAH05378, 1 a.a. - 252 a.a.) full-length recombinant protein with GST.

Alternate Names

C4b binding protein, beta chain, C4b-binding protein beta chain, C4BP, complement component 4 binding protein, beta, complement component 4 binding protein, beta chain, complement component 4-binding protein, beta

Gene Symbol

C4BPB

Additional C4 binding protein B Products

Product Documents for C4 binding protein B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for C4 binding protein B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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