Caldesmon/CALD1 Antibody (24GB4735)

Novus Biologicals | Catalog # NBP3-51677

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4735
View all Caldesmon/CALD1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Caldesmon/CALD1 (Uniprot: Q05682)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caldesmon/CALD1 Antibody (24GB4735)

Caldesmon/CALD1 Antibody (24GB4735)

Flow Cytometry: Caldesmon/CALD1 Antibody (24GB4735)[NBP3-51677] -

Validation of Caldesmon/CALD1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Caldesmon/CALD1 antibody (1:2,000) and analyzed using BD flow cytometer.
Caldesmon/CALD1 Antibody (24GB4735)

Flow Cytometry: Caldesmon/CALD1 Antibody (24GB4735)[NBP3-51677] -

Flow cytometric analysis of Caldesmon/CALD1expression in H9c2 cells using Caldesmon/CALD1 antibody (1:2,000). Green, isotype control; red, caldesmon 1.
Caldesmon/CALD1 Antibody (24GB4735)

Western Blot: Caldesmon/CALD1 Antibody (24GB4735)[NBP3-51677] -

Western blotting analysis using Caldesmon/CALD1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Caldesmon/CALD1 Antibody (24GB4735)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caldesmon/CALD1

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Gene Symbol

CALD1

Additional Caldesmon/CALD1 Products

Product Documents for Caldesmon/CALD1 Antibody (24GB4735)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caldesmon/CALD1 Antibody (24GB4735)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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