CaM Kinase II delta Antibody (1A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00000817-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # 1A8

Format

Azide and BSA Free
View all CaM Kinase II delta Primary Antibodies »

Product Specifications

Immunogen

CAMK2D (AAH32784, 301 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPI

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Rat reported in scientific literature (PMID: 22766271).

Specificity

CAMK2D (1A8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CaM Kinase II delta Antibody (1A8) - Azide and BSA Free

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02]

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02]

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D expression in transfected 293T cell line by CAMK2D monoclonal antibody (M02), clone 1A8.Lane 1: CAMK2D transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (1A8) [H00000817-M02]

Immunocytochemistry/ Immunofluorescence: CaM Kinase II delta Antibody (1A8) [H00000817-M02]

Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of monoclonal antibody to CAMK2D on NIH/3T3 cell. Antibody concentration 10 ug/ml.
Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02]

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02]

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - CAMK2D monoclonal antibody (M02), clone 1A8 Western Blot analysis of CAMK2D expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02]

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02]

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D over-expressed 293 cell line, cotransfected with CAMK2D Validated Chimera RNAi ( Cat # H00000817-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK2D monoclonal antibody (M02) clone 1A8 (Cat # H00000817-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for CaM Kinase II delta Antibody (1A8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Antibody reactivity against recombinant protein and cell lysate for WB. It has also been used for IF, RNAi Validation and ELISA.

Reviewed Applications

Read 2 reviews rated 4 using H00000817-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CaM Kinase II delta

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Four alternatively spliced transcript variants that encode three different isoforms have been characterized to date. Distinct isoforms of this chain have different expression patterns.

Long Name

Calcium/calmodulin-dependent Protein Kinase II delta Subunit

Alternate Names

CAMK2D

Entrez Gene IDs

817 (Human); 108058 (Mouse); 24246 (Rat)

Gene Symbol

CAMK2D

OMIM

607708 (Human)

UniProt

AAH32784 (Human)

Additional CaM Kinase II delta Products

Product Documents for CaM Kinase II delta Antibody (1A8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CaM Kinase II delta Antibody (1A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CaM Kinase II delta Antibody (1A8) - Azide and BSA Free

Customer Reviews for CaM Kinase II delta Antibody (1A8) - Azide and BSA Free (2)

4 out of 5
2 Customer Ratings
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  • CaM Kinase II delta Antibody (1A8)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Heart lysates
    Species: Rat
    Verified Customer | Posted 08/07/2019
    Adult rat heart lysates, reduced and denatured, resolved on 12% SDS-acrylamide gel. Transfer onto 0.22 um nitrocellulose membrane. Block with Rockland blocking solution. incubate membrane overnight at 4C with 1:100 dilution of CaMKII-delta antibody. wash with PBS-0.1% tween 20. incubate for one hour at room temp with 1:10 000 LiCor IRDye 800CW donkey anti-mouse IgG (H+L). Images taken with Odyssey V3.0.
    CaM Kinase II delta Antibody (1A8) - Azide and BSA Free H00000817-M02
  • Name: Anne Frei
    Application: Western Blot
    Sample Tested: Whole cell lysates
    Species: Human
    Verified Customer | Posted 02/11/2015
    whole cell lysates
    CaM Kinase II delta Antibody (1A8) - Azide and BSA Free H00000817-M02

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