CASK Antibody (GT27)

Novus Biologicals | Catalog # NBP3-48791

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # GT27
View all CASK Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to human CASK.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CASK Antibody (GT27)

CASK Antibody (GT27)

Western Blot: CASK Antibody (GT27) [NBP3-48791] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody [GT27] (NBP3-48791) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
CASK Antibody (GT27)

Immunocytochemistry/ Immunofluorescence: CASK Antibody (GT27) [NBP3-48791] -

CASK antibody [GT27] detects CASK protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CASK stained by CASK antibody [GT27] (NBP3-48791) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
CASK Antibody (GT27)

Western Blot: CASK Antibody (GT27) [NBP3-48791] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody [GT27] (NBP3-48791) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for CASK Antibody (GT27)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CASK

Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements.

Alternate Names

Calcium/calmodulin-dependent serine protein kinase, calcium/calmodulin-dependent serine protein kinase (MAGUK family), calcium/calmodulin-dependent serine protein kinase membrane-associatedguanylate kinase, CAMGUK, CMG, EC 2.7.11, EC 2.7.11.1, FGS4CAGH39, FLJ22219, hCASK, LIN2FLJ31914, MICPCH, peripheral plasma membrane protein CASK, Protein lin-2 homolog, TNRC8, trinucleotide repeat containing 8

Gene Symbol

CASK

Additional CASK Products

Product Documents for CASK Antibody (GT27)

Certificate of Analysis

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Product Specific Notices for CASK Antibody (GT27)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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