Key Product Details

Species Reactivity

Mouse

Applications

ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), Neutralization

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG2A Clone # 158132
View all CCL22/MDC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # O88430

Specificity

Detects mouse CCL22/MDC in ELISAs and Western blots. In Western blots, this antibody shows 25% cross-reactivity with recombinant viral MIP‑II and no cross-reactivity with rmCCL1, 2, 3, 4, 6, 7, CCL9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 24, 25, 27, rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, or rrCCL20.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for CCL22/MDC Antibody (158132) [CoraFluor™ 1]

CCL22/MDC Antibody (158132) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for CCL22/MDC Antibody (158132) [CoraFluor™ 1]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CCL22/MDC

Chemokines are a large family of small secreted proteins that regulate migration of white blood cells. Based on the arrangement of the first two of the four conserved cysteine residues, chemokines are classified into four subfamilies, CXC, CC, C and recently identified CX3C (1). The CC chemokines, in which the first 2 cysteines are adjacent, usually act on monocytes, T lymphocytes, and, in some cases, eosinophils, basophils, or mast cells. The CC subfamily proteins are 70 to 100 amino acids long, have 25 to 75% identity with each other, and include novel member of the CC chemokine subfamily, designated ABCD1 (SCYA22). The SCYA22 protein shares 28 to 34% identity with other CC chemokines and contains the characteristic 4-cysteine motif and 9 other highly conserved residues (2). The activated T cell-attracting CC chemokine CCL22 is expressed by stimulated B cells and mature dendritic cells (DC). Both identical and distinct proteins contribute to expression of CCL22 in DC and B cells (3).

Alternate Names

ABCD-1, MDC, STCP-1

Gene Symbol

CCL22

Additional CCL22/MDC Products

Product Documents for CCL22/MDC Antibody (158132) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CCL22/MDC Antibody (158132) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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