CDC7 Antibody (DCS-341) - BSA Free

Novus Biologicals | Catalog # NB120-10535

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # DCS-341

Format

BSA Free
View all CDC7 Primary Antibodies »

Product Specifications

Immunogen

full-length human Cdc7 kinase.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Localization

Nuclear

Specificity

Mouse Monoclonal to Cdc7 Kinase (DCS-341)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDC7 Antibody (DCS-341) - BSA Free

Western Blot: CDC7 Antibody (DCS-341)BSA Free [NB120-10535]

Western Blot: CDC7 Antibody (DCS-341)BSA Free [NB120-10535]

Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.

Applications for CDC7 Antibody (DCS-341) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

0.2 - 0.4 ug/ml
Application Notes
ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Western Blot: Use at a concentration of 0.4 ug/ml. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reviewed Applications

Read 1 review rated 4 using NB120-10535 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC7

DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Long Name

Cell division cycle 7-related protein kinase

Alternate Names

CDC7L1, HsCdc7, huCdc7

Entrez Gene IDs

8317 (Human)

Gene Symbol

CDC7

Additional CDC7 Products

Product Documents for CDC7 Antibody (DCS-341) - BSA Free

Certificate of Analysis

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Product Specific Notices for CDC7 Antibody (DCS-341) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CDC7 Antibody (DCS-341) - BSA Free

Customer Reviews for CDC7 Antibody (DCS-341) - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell
    Species: Human
    Verified Customer | Posted 10/04/2015

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