CDK2 [p Tyr15] Antibody (7Z5H5)

Name: CDK2 [p Tyr15] Antibody (7Z5H5)
Brand: Novus Biologicals
SKU: NBP3-33556
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-33556

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 7Z5H5

View all CDK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y15 of human CDK2 (NP_001789.2).

Sequence:
GTYGV

Modification

p Tyr15

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDK2 [p Tyr15] Antibody (7Z5H5)

Western Blot: CDK2 [p Tyr15] Antibody (7Z5H5) [NBP3-33556] -

Western Blot: CDK2 [p Tyr15] Antibody (7Z5H5) [NBP3-33556] - Western blot analysis of lysates from NIH/3T3 cells, using CDK2 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3s.

CDK2 [p Tyr15] Antibody (7Z5H5) [NBP3-33556] -

Analysis of lysates from HeLa cells using Phospho-CDK2-Y15 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with Hydroxyurea (4 Mm) at 37℃ for 20 hours at 37℃ for 24 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit.

Applications for CDK2 [p Tyr15] Antibody (7Z5H5)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:1000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 [p Tyr15] Antibody (7Z5H5)

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Certificate of Analysis

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Product Specific Notices for CDK2 [p Tyr15] Antibody (7Z5H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

Intracellular Kinases

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