CENPC Antibody (5E3K5)

Name: CENPC Antibody (5E3K5)
Brand: Novus Biologicals
SKU: NBP3-16305
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16305

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5E3K5 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 380-520 of human CENPC (Q03188). VLDTSYALIGETVNNYRSTKYEMYSKNAEKPSRSKRTIKQKQRRKFMAKPAEEQLDVGQSKDENIHTSHITQDEFQRNSDRNMEEHEEMGNDCVSKKQMPPVGSKKSSTRKDKEESKKKRFSSESKNKLVPEEVTSTVTKS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CENPC Antibody (5E3K5)

Western Blot: CENPC Antibody (5E3K5) [NBP3-16305]

Western Blot: CENPC Antibody (5E3K5) [NBP3-16305] - Western blot analysis of extracts of various cell lines, using CENPC Rabbit mAb (NBP3-16305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/ Immunofluorescence: CENPC Antibody (5E3K5) [NBP3-16305] -

Immunocytochemistry/ Immunofluorescence: CENPC Antibody (5E3K5) [NBP3-16305] - Immunofluorescence analysis of C6 cells using CENPC Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for CENPC Antibody (5E3K5)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CENPC

Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12. [provided by RefSeq]

Alternate Names

CENP-C, CENP-C 1, CENPCInterphase centromere complex protein 7, Centromere autoantigen C, centromere autoantigen C1, centromere protein C 1, hcp-4, ICEN7, MIF2

Gene Symbol

CENPC

Additional CENPC Products

Product Documents for CENPC Antibody (5E3K5)

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Certificate of Analysis

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Product Specific Notices for CENPC Antibody (5E3K5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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