CNOT4 Antibody (OTI3D11) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71203

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D11

Format

Azide and BSA Free
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Product Specifications

Immunogen

Human recombinant protein fragment corresponding to ammino acids 190-455 of human CNOT4(NP_037448) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

70.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CNOT4 Antibody (OTI3D11) - Azide and BSA Free

Western Blot: CNOT4 Antibody (OTI3D11)Azide and BSA Free [NBP2-71203]

Western Blot: CNOT4 Antibody (OTI3D11)Azide and BSA Free [NBP2-71203]

Western Blot: CNOT4 Antibody (OTI3D11) - Azide and BSA Free [NBP2-71203] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow Cytometry: CNOT4 Antibody (OTI3D11) - Azide and BSA Free [NBP2-71203]

Flow Cytometry: CNOT4 Antibody (OTI3D11) - Azide and BSA Free [NBP2-71203]

Flow Cytometry: CNOT4 Antibody (OTI3D11) - Azide and BSA Free [NBP2-71203] - Analysis of Jurkat cells, using CNOT4 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: CNOT4 Antibody (OTI3D11) - Azide and BSA Free [NBP2-71203]

Flow Cytometry: CNOT4 Antibody (OTI3D11) - Azide and BSA Free [NBP2-71203]

Flow Cytometry: CNOT4 Antibody (OTI3D11) - Azide and BSA Free [NBP2-71203] - Analysis of Hela cells, using CNOT4 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Applications for CNOT4 Antibody (OTI3D11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CNOT4

Alternate Names

CCR4-associated factor 4, CCR4-NOT transcription complex, subunit 4, E3 ubiquitin-protein ligase CNOT4, EC 6.3.2.-, NOT4yeast) homolog

Gene Symbol

CNOT4

Additional CNOT4 Products

Product Documents for CNOT4 Antibody (OTI3D11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CNOT4 Antibody (OTI3D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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