Complement C7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38143

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 22-270 of human Complement C7 (NP_000578.2).

Sequence:
RSVAVYGQYGGQPCVGNAFETQSCEPTRGCPTEEGCGERFRCFSGQCISKSLVCNGDSDCDEDSADEDRCEDSERRPSCDIDKPPPNIELTGNGYNELTGQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Complement C7 Antibody - BSA Free

Complement C7 Antibody

Immunocytochemistry/ Immunofluorescence: Complement C7 Antibody [NBP3-38143] -

Immunocytochemistry/ Immunofluorescence: Complement C7 Antibody [NBP3-38143] - Immunofluorescence analysis of HeLa cells using Complement C7 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Complement C7 Antibody

Western Blot: Complement C7 Antibody [NBP3-38143] -

Western Blot: Complement C7 Antibody [NBP3-38143] - Western blot analysis of various lysates using Complement C7 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Complement C7 Antibody

Immunocytochemistry/ Immunofluorescence: Complement C7 Antibody [NBP3-38143] -

Immunocytochemistry/ Immunofluorescence: Complement C7 Antibody [NBP3-38143] - Immunofluorescence analysis of NIH/3T3 cells using Complement C7 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Complement C7 Antibody

Western Blot: Complement C7 Antibody [NBP3-38143] -

Western Blot: Complement C7 Antibody [NBP3-38143] - Western blot analysis of lysates from Mouse plasma, using Complement C7 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Applications for Complement C7 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Complement C7

C7 is a component of the complement system. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection.

Alternate Names

complement component 7, complement component C7

Gene Symbol

C7

Additional Complement C7 Products

Product Documents for Complement C7 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Complement C7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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