Cyclooxygenase (COX), also known as prostaglandin G/H synthase, is a membrane bound enzyme partly responsible for the catalysis of prostanoid synthesis. COX is expressed as at least three different isoforms. COX-1 is constitutively expressed and thought to regulate a number of 'housekeeping' functions such as vascular hemostasis, renal blood flow, and glomerular function. COX-2 expression is tightly regulated and induced by inflammatory mediators such as growth factors, cytokines, and endotoxin. COX-3 appears to be much more strictly regulated spatially and is observed in greatest abundance in cerebral cortex.
COX-1 Antibody (345305) [Janelia Fluor® 669]
Novus Biologicals | Catalog # FAB3740JF669
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Janelia Fluor 669
Antibody Source
Monoclonal Rat IgG2A Clone # 345305
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Product Specifications
Immunogen
E. coli-derived recombinant human COX-1
Ala24-Leu599
Accession # P23219
Ala24-Leu599
Accession # P23219
Specificity
Detects human COX-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Semaphorin 3A, 3B, 6B, 6C, 6D, recombinant mouse Semaphorin 3A, 3B, 3E, 3F, 6A, or 7A is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for COX-1 Antibody (345305) [Janelia Fluor® 669]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: COX-1
Long Name
Cyclooxygenase 1
Alternate Names
COX1, PTGS1
Gene Symbol
PTGS1
Additional COX-1 Products
Product Documents for COX-1 Antibody (345305) [Janelia Fluor® 669]
Product Specific Notices for COX-1 Antibody (345305) [Janelia Fluor® 669]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for COX-1 Antibody (345305) [Janelia Fluor® 669]
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A: Unfortunately we do not currently have any COX1 recombinant proteins that are expected to be active.
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