CRYBA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33010

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Predicted:

Bovine (93%), Canine (96%), Guinea Pig (95%), Rabbit (96%), Rat (95%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CRYBA1 Primary Antibodies »

Product Specifications

Immunogen

Full length human beta A3 Crystallin Recombinant protein.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 31488069).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRYBA1 Antibody - BSA Free

Western Blot: CRYBA1 Antibody [NBP1-33010]

Western Blot: CRYBA1 Antibody [NBP1-33010]

CRYBA1-Antibody-Western-Blot-NBP1-33010-img0005.jpg
Western Blot: CRYBA1 Antibody [NBP1-33010]

Western Blot: CRYBA1 Antibody [NBP1-33010]

Western Blot: CRYBA1 Antibody [NBP1-33010] - A. 50 ug mouse fetal eye lysate/extract 12 % SDS-PAGE beta A3 Crystallin antibody dilution: 1:10000
CRYBA1 Antibody

Immunocytochemistry/ Immunofluorescence: CRYBA1 Antibody [NBP1-33010] -

Immunocytochemistry/ Immunofluorescence: CRYBA1 Antibody [NBP1-33010] - Immunofluorescence staining of exogenous WT & mutated CRYBA1 in 2 cell lines. After the WT & deleted forms of CRYBA1 cDNA constructs were transfected into 293 T & SRA lines, cells were stained with FLAG primary antibody. p.G91del mutation led to greater aggregation of the protein at the cell membrane. Blue: nuclear; green: exogenous CRYBA1, scale bar: 20 μM Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31488069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CRYBA1 Antibody

Western Blot: CRYBA1 Antibody [NBP1-33010] -

Western Blot: CRYBA1 Antibody [NBP1-33010] - CRYBA1 is expressed less in cataract lens epithelium. (a) Western blot images of CRYBA1 in ARC & normal control lens epithelium. Three samples from each group are presented. beta -Actin was used as an internal control. (b) Quantification of the gel images of the Western blots in (a) (n = 3). (c) The mRNA level of CRYBA1 was measured by qPCR in ARC & normal control lens epithelium. beta -Actin was used as an internal control (n = 3). Student’s t test, *P < 0.05, **P < 0.01 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31488069), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CRYBA1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:31488069).

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRYBA1

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.

Alternate Names

CRYB1beta-crystallin A3, crystallin, beta A1, crystallin, beta A3, eye lens structural protein

Gene Symbol

CRYBA1

Additional CRYBA1 Products

Product Documents for CRYBA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CRYBA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for CRYBA1 Antibody - BSA Free

Customer Reviews for CRYBA1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review CRYBA1 Antibody - BSA Free and earn rewards!

Have you used CRYBA1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies