CXC chemokine ligand 16 (CXCL16) is a type I membrane protein that has a non-ELR CXC chemokine domain in its extracellular region. Fractalkine (CX3CL1) and CXCL16 constitute the only two transmembrane chemokines. Mouse and human CXCL16 share 70% amino acid sequence similarity within their chemokine domains and 49% overall amino acid sequence identity.
CXCL16 Antibody (4.4) - BSA Free
Novus Biologicals | Catalog # NBP2-50187
![Western Blot: CXCL16 Antibody (4.4)BSA Free [NBP2-50187]](https://resources.rndsystems.com/images/products/CXCL16-Antibody-4-4-Western-Blot-NBP2-50187-img0001.jpg "Western Blot: CXCL16 Antibody (4.4)BSA Free [NBP2-50187]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4.4
Format
BSA Free
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Product Specifications
Immunogen
GST-CXCL16 (143-224aa) fusion protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CXCL16 Antibody (4.4) - BSA Free
Western Blot: CXCL16 Antibody (4.4)BSA Free [NBP2-50187]
Western Blot: CXCL16 Antibody (4.4) [NBP2-50187] - Analysis of lysates prepared from HEK293 FT cells with a plasmid expressing the full length CXCL16 open reading frame. Lane 1: 293FT transfected cell lysate probed with anti-CXCL16 [4.4], Lane 2: 293FT mock cell lysate probed with the same antibody.![Immunocytochemistry/ Immunofluorescence: CXCL16 Antibody (4.4) - BSA Free [NBP2-50187]](https://resources.rndsystems.com/images/products/CXCL16-Antibody-4-4-Immunocytochemistry-Immunofluorescence-NBP2-50187-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CXCL16 Antibody (4.4) - BSA Free [NBP2-50187]")
Immunocytochemistry/ Immunofluorescence: CXCL16 Antibody (4.4) - BSA Free [NBP2-50187]
Immunocytochemistry/Immunofluorescence: CXCL16 Antibody (4.4) [NBP2-50187] - Staining of cos7 cells transfected with a plasmid containing a full-length CXCL16 open reading frame (a). 48 hours post transfection cells were fixed with 4% paraformaldehyde permeabilised with 0.1% Triton X-100 and stained using Anti-CXCL16 [4.4] and followed by secondary goat anti-mouse FITC (1:100). Nucleic acid DAPI counterstain is shown in blue. A negative control mock is also shown (b).Applications for CXCL16 Antibody (4.4) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:100 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CXCL16
Alternate Names
CXCL16
Gene Symbol
CXCL16
Additional CXCL16 Products
Product Documents for CXCL16 Antibody (4.4) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CXCL16 Antibody (4.4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
