CYTIP Antibody

Novus Biologicals | Catalog # NB100-1002

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence SLQRLLQHSSNGN-C corresponding to N-Terminus according to NP_004279.3.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CYTIP Antibody

Western Blot: CYTIP Antibody [NB100-1002]

Western Blot: CYTIP Antibody [NB100-1002]

Western Blot: CYTIP Antibody [NB100-1002] - Staining of Jurkat (A) and (1 ug/mL) MOLT4 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunofluorescence: CYTIP Antibody [NB100-1002]

Immunofluorescence: CYTIP Antibody [NB100-1002]

Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunofluorescence: CYTIP Antibody [NB100-1002]

Immunofluorescence: CYTIP Antibody [NB100-1002]

Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong cytoplasmic and weak nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for CYTIP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx 38 kDa band observed in lysates of cell line Jurkat and approx 40 kDa in lysates of cell line MOLT4 (calculated MW of 40 kDa according to NP_004279.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CYTIP

This protein contains 2 leucine zipper domains and a putative C-terminal nuclear targeting signal, and it does not have any hydrophobic regions. It is expressed weakly in resting NK and T cells. It and cytohesin-1 physically interact through their coiled-coil domains. Cytohesin-1, in addition to its role in cell adhesion, is a guanine nucleotide-exchange protein activator of ARF GTPases.

Alternate Names

B3-1, CASPCytohesin-associated scaffolding protein, Cbp HE, CYBRCytohesin-binding protein HE, CYTHIP, cytohesin 1 interacting protein, Cytohesin binder and regulator, cytohesin-1 interacting protein, cytohesin-interacting protein, HEPleckstrin homology Sec7 and coiled-coil domains-binding protein, pleckstrin homology, Sec7 and coiled/coil domains, binding protein, pleckstrin homology, Sec7 and coiled-coil domains, binding protein, PSCDBPcytohesin binding protein HE

Entrez Gene IDs

9595 (Human)

Gene Symbol

CYTIP

UniProt

NP_004279. (Human)

Additional CYTIP Products

Product Documents for CYTIP Antibody

Certificate of Analysis

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Product Specific Notices for CYTIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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