DOT1L Antibody (6A6) - Azide and BSA Free

Novus Biologicals | Catalog # H00084444-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Feline

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A6

Format

Azide and BSA Free
View all DOT1L Primary Antibodies »

Product Specifications

Immunogen

DOT1L (NP_115871, 3 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLR

Reactivity Notes

Human. Other species not tested.

Specificity

DOT1L - DOT1-like, histone H3 methyltransferase (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DOT1L Antibody (6A6) - Azide and BSA Free

Western Blot: DOT1L Antibody (6A6) [H00084444-M01]

Western Blot: DOT1L Antibody (6A6) [H00084444-M01]

Western Blot: DOT1L Antibody (6A6) [H00084444-M01] - DOT1L monoclonal antibody (M01), clone 6A6. Analysis of DOT1L expression in LNCaP.
Immunocytochemistry/ Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01]

Immunocytochemistry/ Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01]

Immunocytochemistry/Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01] - Analysis of monoclonal antibody to DOT1L on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: DOT1L Antibody (6A6) [H00084444-M01] - Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody.

Applications for DOT1L Antibody (6A6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Entrez Gene IDs

84444 (Human)

Gene Symbol

DOT1L

OMIM

607375 (Human)

UniProt

NP_115871 (Human)

Additional DOT1L Products

Product Documents for DOT1L Antibody (6A6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DOT1L Antibody (6A6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for DOT1L Antibody (6A6) - Azide and BSA Free

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Protocols

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FAQs

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