DUSP5 Antibody (2F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00001847-M04

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2F3

Format

Azide and BSA Free
View all DUSP5 Primary Antibodies »

Product Specifications

Immunogen

DUSP5 (NP_004410, 286 a.a. ~ 384 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LKEAFDYIKQRRSMVSPNFGFMGQLLQYESEILPSTPNPQPPSCQGEAAGSSLIGHLQTLSPDMQGAYCTFPASVLAPVPTHSTVSELSRSPVATATSC

Reactivity Notes

Rat reported in scientific literature (PMID: 25397684).

Specificity

DUSP5 - dual specificity phosphatase 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DUSP5 Antibody (2F3) - Azide and BSA Free

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04]

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04]

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of DUSP5 expression in transfected 293T cell line by DUSP5 monoclonal antibody (M04), clone 2F3. Lane 1: DUSP5 transfected lysate (Predicted MW: 42.1 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: DUSP5 Antibody (2F3) [H00001847-M04]

Immunocytochemistry/ Immunofluorescence: DUSP5 Antibody (2F3) [H00001847-M04]

Immunocytochemistry/Immunofluorescence: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of monoclonal antibody to DUSP5 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: DUSP5 Antibody (2F3) [H00001847-M04]

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04]

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Western Blot analysis of DUSP5 expression in PC-12 ( Cat # L012V1 ).
Western Blot: DUSP5 Antibody (2F3) [H00001847-M04]

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04]

Western Blot: DUSP5 Antibody (2F3) [H00001847-M04] - Analysis of DUSP5 expression in K-562 (Cat # L009V1).
ELISA: DUSP5 Antibody (2F3) [H00001847-M04]

ELISA: DUSP5 Antibody (2F3) [H00001847-M04]

ELISA: DUSP5 Antibody (2F3) [H00001847-M04] - Detection limit for recombinant GST tagged DUSP5 is approximately 1ng/ml as a capture antibody.

Applications for DUSP5 Antibody (2F3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DUSP5

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq]

Alternate Names

dual specificity phosphatase 5, dual specificity protein phosphatase 5, Dual specificity protein phosphatase hVH3, DUSP, EC 3.1.3.16, EC 3.1.3.48, HVH3VH1-like phosphatase 3, serine/threonine specific protein phosphatase, VH3

Entrez Gene IDs

1847 (Human)

Gene Symbol

DUSP5

OMIM

603069 (Human)

UniProt

NP_004410 (Human)

Additional DUSP5 Products

Product Documents for DUSP5 Antibody (2F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DUSP5 Antibody (2F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DUSP5 Antibody (2F3) - Azide and BSA Free

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Protocols

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FAQs

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