EEA1 Antibody (2G2) - Azide and BSA Free

Novus Biologicals | Catalog # H00008411-M03

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Key Product Details

Species Reactivity

Human, Mouse, Hamster

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G2

Format

Azide and BSA Free
View all EEA1 Primary Antibodies »

Product Specifications

Immunogen

EEA1 (NP_003557, 1312 a.a. ~ 1411 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 25419208).

Marker

Early Endosome Marker

Specificity

EEA1 - early endosome antigen 1, 162kD (2G2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EEA1 Antibody (2G2) - Azide and BSA Free

Western Blot: EEA1 Antibody (2G2) [H00008411-M03]

Western Blot: EEA1 Antibody (2G2) [H00008411-M03]

Western Blot: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of EEA1 expression in A-431 (Cat # L015V1).
Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03]

Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03]

Immunocytochemistry/Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of monoclonal antibody to EEA1 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: EEA1 Antibody (2G2) [H00008411-M03]

ELISA: EEA1 Antibody (2G2) [H00008411-M03]

ELISA: EEA1 Antibody (2G2) [H00008411-M03] - Detection limit for recombinant GST tagged EEA1 is approximately 0.03ng/ml as a capture antibody.

Applications for EEA1 Antibody (2G2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EEA1

EEA1 binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking

Long Name

Early Endosome Antigen 1

Alternate Names

MST105, MSTP105, ZFYVE2

Entrez Gene IDs

8411 (Human)

Gene Symbol

EEA1

UniProt

NP_003557

Additional EEA1 Products

Product Documents for EEA1 Antibody (2G2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EEA1 Antibody (2G2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EEA1 Antibody (2G2) - Azide and BSA Free

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Protocols

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FAQs

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