EEF2K Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92950

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EEF2K Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1). KLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EEF2K Antibody - BSA Free

Western Blot: EEF2K AntibodyBSA Free [NBP2-92950]

Western Blot: EEF2K AntibodyBSA Free [NBP2-92950]

Western Blot: EEF2K Antibody [NBP2-92950] - Analysis of extracts of various cell lines, using EEF2K at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 40s.
Immunocytochemistry/ Immunofluorescence: EEF2K Antibody - BSA Free [NBP2-92950]

Immunocytochemistry/ Immunofluorescence: EEF2K Antibody - BSA Free [NBP2-92950]

Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NBP2-92950] - Analysis of A-549 cells using EEF2K. Blue: DAPI for nuclear staining.
Knockout Validated: EEF2K Antibody - BSA Free [NBP2-92950]

Western Blot: EEF2K Antibody - BSA Free [NBP2-92950]

Western Blot: EEF2K Antibody [NBP2-92950] - Analysis of extracts from normal (control) and EEF2K knockout (KO) HeLa cells, using EEF2K antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit

Applications for EEF2K Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EEF2K

EEF2K encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Alternate Names

Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, EC 2.7.11, EC 2.7.11.20, eEF-2 kinase, eEF-2Kcalmodulin-dependent protein kinase III, elongation factor-2 kinase, eukaroytic elongation factor 2 kinase, eukaryotic elongation factor 2 kinase, eukaryotic elongation factor-2 kinase, HSU93850, MGC45041

Gene Symbol

EEF2K

Additional EEF2K Products

Product Documents for EEF2K Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EEF2K Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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