EHD2 Antibody (2D8) - Azide and BSA Free

Novus Biologicals | Catalog # H00030846-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D8

Format

Azide and BSA Free
View all EHD2 Primary Antibodies »

Product Specifications

Immunogen

EHD2 (NP_055416, 365 a.a. ~ 427 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AHDFTKFHSLKPKLLEALDEMLTHDIAKLMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGP

Reactivity Notes

Human. Other species not tested.

Localization

Nuclear

Specificity

EHD2 - EH-domain containing 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for EHD2 Antibody (2D8) - Azide and BSA Free

Western Blot: EHD2 Antibody (2D8) [H00030846-M01]

Western Blot: EHD2 Antibody (2D8) [H00030846-M01]

Western Blot: EHD2 Antibody (2D8) [H00030846-M01] - EHD2 monoclonal antibody (M01), clone 2D8 Analysis of EHD2 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: EHD2 Antibody (2D8) [H00030846-M01]

Immunocytochemistry/ Immunofluorescence: EHD2 Antibody (2D8) [H00030846-M01]

Immunocytochemistry/Immunofluorescence: EHD2 Antibody (2D8) [H00030846-M01] - Analysis of monoclonal antibody to EHD2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: EHD2 Antibody (2D8) [H00030846-M01]

Western Blot: EHD2 Antibody (2D8) [H00030846-M01]

Western Blot: EHD2 Antibody (2D8) [H00030846-M01] - Analysis of EHD2 expression in transfected 293T cell line by EHD2 monoclonal antibody (M01), clone 2D8.Lane 1: EHD2 transfected lysate(61.2 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: EHD2 Antibody (2D8) [H00030846-M01] - Detection limit for recombinant GST tagged EHD2 is approximately 0.03ng/ml as a capture antibody.

Applications for EHD2 Antibody (2D8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EHD2

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Alternate Names

EH domain containing 2, EH domain-containing protein 2, EH-domain containing 2, FLJ96617, PAST homolog 2, PAST2

Entrez Gene IDs

30846 (Human)

Gene Symbol

EHD2

OMIM

605890 (Human)

UniProt

NP_055416 (Human)

Additional EHD2 Products

Product Documents for EHD2 Antibody (2D8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for EHD2 Antibody (2D8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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