Endothelial Lipase Antibody - BSA Free

Novus Biologicals | Catalog # NB400-118

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative)

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Endothelial Lipase Primary Antibodies »

Product Specifications

Immunogen

An N-terminal synthetic peptide made to human Endothelial Lipase [UniProt# Q9Y5X9]

Reactivity Notes

Does not appear to recognize mouse.

Localization

Secreted, cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Endothelial Lipase Antibody - BSA Free

Western Blot: Endothelial Lipase Antibody [NB400-118]

Western Blot: Endothelial Lipase Antibody [NB400-118]

Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)
Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody [NB400-118]

Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody [NB400-118]

Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-118] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: Endothelial Lipase Antibody [NB400-118]

Western Blot: Endothelial Lipase Antibody [NB400-118]

Western Blot: Endothelial Lipase Antibody [NB400-118] - Analysis of Endothelial Lipase in ECV304 cells.

Applications for Endothelial Lipase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000. Use reported in scientific literature (PMID 22383927)

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500
Application Notes
In Western blot this antibody recognizes a band at ~57 kDa, representing the mature, non-glycosylated form of endothelial lipase protein. Another band is seen at ~ 45 kDa, representing the proteolytic form of the protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Endothelial Lipase

Endothelial lipase (EL) is an essential element of HDL metabolism which catalyzes the hydrolysis of HDL-phospholipids for facilitating their clearance from circulation. EL is synthesized in endothelial cells, however, is also expressed in other cell types such as coronary artery endothelial cells, umbilical vein endothelial cells, hepatocytes, osteosarcoma cell lines etc. with highest levels seen in organs including liver, placenta, lung, thyroid, kidney, testis and corpus luteum of ovary. EL is synthesized as a 55 kD protein, secreted as a 68 kD mature protein and after secretion, a portion of EL is cleaved and inactivated by convertases. Uncleaved EL is bound to heparan-sulfate proteoglycans on the cell surfaces where via its bridging functions, it facilitates HDL particle binding/uptake and selective uptake of HDL cholesteryl esters. Through its marked sn-1 phospholipase activity, EL cleaves lipoprotein-associated phospholipids, primarily HDL-phosphatidylethanolamine (HDL-PE) and -phosphatidylcholine (HDL-PC), generating mostly saturated free fatty acids and sn-1 lyso sn-2 acyl lysophospholipids (LysoPL). By its rather weak lysophospholipase activity, EL further hydrolyzes some of those EL-generated LPL, liberating unsaturated FFA. EL expression increases in cultured endothelial cells exposed to interleukin-1 beta or TNF-alpha as well as in vivo upon LPS exposure suggesting its role in inflammatory conditions. Accordingly, an elevated EL levels are found in patients with subclinical inflammation as encountered in the metabolic syndrome and coronary atherosclerosis.

Alternate Names

EC 3.1.1, EC 3.1.1.3, EDLendothelial lipase, ELlipoprotein lipase H, Endothelial cell-derived lipase, lipase, endothelial, PRO719

Entrez Gene IDs

9388 (Human); 16890 (Mouse)

Gene Symbol

LIPG

UniProt

Q9Y5X9 (Human)

Additional Endothelial Lipase Products

Product Documents for Endothelial Lipase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Endothelial Lipase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Endothelial Lipase Antibody - BSA Free

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Protocols

View specific protocols for Endothelial Lipase Antibody - BSA Free (NB400-118):

Endothelial Lipase Antibody:
Western Blot Procedure

1. Heat diluted samples at 70 degrees Celcius for 10 minutes.
2. Load samples onto a 10% Bis-Tris gel.
3. Run gel for ~45 minutes at 200V.
4. Transfer gel to PVDF membrane at 45V for ~90 minutes.
5. Block the membrane overnight at 4 degrees Celcius, in blocking buffer [PBS + 0.2% Tween-20 + 5% milk], gently shaking.
6. Rinse the membrane twice, quickly, in PBS-T.
7. Wash the membrane in PBS-T 3 times for 5 minutes, each.
8. Incubate the membrane with primary antibody [NB 400-111 or NB 400-118], diluted 1:500 in blocking buffer, for 1 hour at RT, gently shaking.
9. Rinse the membrane twice, quickly, in PBS-T.
10. Wash the membrane in PBS-T 3 times for 5 minutes, each.
11. Incubate the membrane with secondary antibody [NB 730-H], diluted 1:5,000 in blocking buffer, for 1 hour at RT, gently shaking.
12. Rinse the membrane twice, quickly, in PBS-T.
13. Wash the membrane in PBS-T 3 times for 5 minutes, each.
14. Detect protein using ECL, ~1 minute exposure.

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