EphB2 Antibody (2D12C6)

Novus Biologicals | Catalog # NBP1-47525

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Key Product Details

Species Reactivity

Human, Porcine

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2D12C6
View all EphB2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.

Reactivity Notes

Porcine reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for EphB2 Antibody (2D12C6)

Western Blot: EphB2 Antibody (2D12C6)BSA Free [NBP1-47525]

Western Blot: EphB2 Antibody (2D12C6)BSA Free [NBP1-47525]

Western Blot: EphB2 Antibody (2D12C6) [NBP1-47525] - Analysis using EphB2 mouse mAb against truncated EphB2 recombinant protein (1) and extracellular EphB2(aa19-476)-hIgGFc transfected CHO-K1 cell lysate(2).
Immunocytochemistry/ Immunofluorescence: EphB2 Antibody (2D12C6) - BSA Free [NBP1-47525]

Immunocytochemistry/ Immunofluorescence: EphB2 Antibody (2D12C6) - BSA Free [NBP1-47525]

Immunocytochemistry/Immunofluorescence: EphB2 Antibody (2D12C6) [NBP1-47525] - Analysis of porcine outgrowth endothelial cells isolated from fetal porcine blood cord (pOECs) using EphB2 antibody. Primary antibody dilution 1:100. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: EphB2 Antibody (2D12C6) - BSA Free [NBP1-47525]

Immunocytochemistry/ Immunofluorescence: EphB2 Antibody (2D12C6) - BSA Free [NBP1-47525]

Immunocytochemistry/Immunofluorescence: EphB2 Antibody (2D12C6) [NBP1-47525] - Analysis of Hela (left) and HepG2 (right) cells using EphB2 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Applications for EphB2 Antibody (2D12C6)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 1 review rated 4 using NBP1-47525 in the following applications:

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphB2

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Long Name

Eph Receptor B2

Alternate Names

Cek5, Drt, Erk, Hek5, Nuk, Qek2, Sek3, Tyro5

Entrez Gene IDs

2048 (Human)

Gene Symbol

EPHB2

Additional EphB2 Products

Product Documents for EphB2 Antibody (2D12C6)

Certificate of Analysis

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Product Specific Notices for EphB2 Antibody (2D12C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for EphB2 Antibody (2D12C6) (1)

4 out of 5
1 Customer Rating
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4 Stars
100%
3 Stars
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1 Stars
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Customer Images

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  • EphB2 Antibody (2D12C6)
    Name: Sandra Meliton Barbancho
    Application: Immunofluorence in 2D culture
    Sample Tested: porcine endothelial cells
    Species: Porcine
    Verified Customer | Posted 12/02/2022
    images got by Olympus microscope
    Porcine endothelial cells isolated from fetal blood cord
    EphB2 Antibody (2D12C6) NBP1-47525
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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